Human metapneumovirus infection induces significant changes in small noncoding rna expression in airway epithelial cells

Junfang Deng, Ryan N. Ptashkin, Qingrong Wang, Guangliang Liu, Guanping Zhang, Inhan Lee, Yong Sun Lee, Xiaoyong Bao

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Small noncoding RNAs (sncRNAs), such as microRNAs (miRNA), virus-derived sncRNAs, and more recently identified tRNA-derived RNA fragments, are critical to posttranscriptional control of genes. Upon viral infection, host cells alter their sncRNA expression as a defense mechanism, while viruses can circumvent host defenses and promote their own propagation by affecting host cellular sncRNA expression or by expressing viral sncRNAs. Therefore, characterizing sncRNA profiles in response to viral infection is an important tool for understanding host-virus interaction, and for antiviral strategy development. Human metapneumovirus (hMPV), a recently identified pathogen, is a major cause of lower respiratory tract infections in infants and children. To investigate whether sncRNAs play a role in hMPV infection, we analyzed the changes in sncRNA profiles of airway epithelial cells in response to hMPV infection using ultrahigh-throughput sequencing. Of the cloned sncRNAs, miRNA was dominant in A549 cells, with the percentage of miRNA increasing in a time-dependent manner after the infection. In addition, several hMPV-derived sncRNAs and corresponding ribonucleases for their biogenesis were identified. hMPV M2-2 protein was revealed to be a key viral protein regulating miRNA expression. In summary, this study revealed several novel aspects of hMPV-mediated sncRNA expression, providing a new perspective on hMPV-host interactions.

Original languageEnglish (US)
Article numbere163
JournalMolecular Therapy - Nucleic Acids
Volume3
DOIs
StatePublished - May 20 2014

Fingerprint

Metapneumovirus
Small Untranslated RNA
Epithelial Cells
Infection
MicroRNAs
Virus Diseases
Viruses
Viral Proteins
Ribonucleases
Transfer RNA
Respiratory Tract Infections
Antiviral Agents

Keywords

  • Human metapneumovirus
  • Human metapneumovirus-derived snRNAs
  • MiRNAs
  • SncRNA

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

Cite this

Human metapneumovirus infection induces significant changes in small noncoding rna expression in airway epithelial cells. / Deng, Junfang; Ptashkin, Ryan N.; Wang, Qingrong; Liu, Guangliang; Zhang, Guanping; Lee, Inhan; Lee, Yong Sun; Bao, Xiaoyong.

In: Molecular Therapy - Nucleic Acids, Vol. 3, e163, 20.05.2014.

Research output: Contribution to journalArticle

Deng, Junfang ; Ptashkin, Ryan N. ; Wang, Qingrong ; Liu, Guangliang ; Zhang, Guanping ; Lee, Inhan ; Lee, Yong Sun ; Bao, Xiaoyong. / Human metapneumovirus infection induces significant changes in small noncoding rna expression in airway epithelial cells. In: Molecular Therapy - Nucleic Acids. 2014 ; Vol. 3.
@article{719252103d2644939e746b9150a5488d,
title = "Human metapneumovirus infection induces significant changes in small noncoding rna expression in airway epithelial cells",
abstract = "Small noncoding RNAs (sncRNAs), such as microRNAs (miRNA), virus-derived sncRNAs, and more recently identified tRNA-derived RNA fragments, are critical to posttranscriptional control of genes. Upon viral infection, host cells alter their sncRNA expression as a defense mechanism, while viruses can circumvent host defenses and promote their own propagation by affecting host cellular sncRNA expression or by expressing viral sncRNAs. Therefore, characterizing sncRNA profiles in response to viral infection is an important tool for understanding host-virus interaction, and for antiviral strategy development. Human metapneumovirus (hMPV), a recently identified pathogen, is a major cause of lower respiratory tract infections in infants and children. To investigate whether sncRNAs play a role in hMPV infection, we analyzed the changes in sncRNA profiles of airway epithelial cells in response to hMPV infection using ultrahigh-throughput sequencing. Of the cloned sncRNAs, miRNA was dominant in A549 cells, with the percentage of miRNA increasing in a time-dependent manner after the infection. In addition, several hMPV-derived sncRNAs and corresponding ribonucleases for their biogenesis were identified. hMPV M2-2 protein was revealed to be a key viral protein regulating miRNA expression. In summary, this study revealed several novel aspects of hMPV-mediated sncRNA expression, providing a new perspective on hMPV-host interactions.",
keywords = "Human metapneumovirus, Human metapneumovirus-derived snRNAs, MiRNAs, SncRNA",
author = "Junfang Deng and Ptashkin, {Ryan N.} and Qingrong Wang and Guangliang Liu and Guanping Zhang and Inhan Lee and Lee, {Yong Sun} and Xiaoyong Bao",
year = "2014",
month = "5",
day = "20",
doi = "10.1038/mtna.2014.18",
language = "English (US)",
volume = "3",
journal = "Molecular Therapy - Nucleic Acids",
issn = "2162-2531",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Human metapneumovirus infection induces significant changes in small noncoding rna expression in airway epithelial cells

AU - Deng, Junfang

AU - Ptashkin, Ryan N.

AU - Wang, Qingrong

AU - Liu, Guangliang

AU - Zhang, Guanping

AU - Lee, Inhan

AU - Lee, Yong Sun

AU - Bao, Xiaoyong

PY - 2014/5/20

Y1 - 2014/5/20

N2 - Small noncoding RNAs (sncRNAs), such as microRNAs (miRNA), virus-derived sncRNAs, and more recently identified tRNA-derived RNA fragments, are critical to posttranscriptional control of genes. Upon viral infection, host cells alter their sncRNA expression as a defense mechanism, while viruses can circumvent host defenses and promote their own propagation by affecting host cellular sncRNA expression or by expressing viral sncRNAs. Therefore, characterizing sncRNA profiles in response to viral infection is an important tool for understanding host-virus interaction, and for antiviral strategy development. Human metapneumovirus (hMPV), a recently identified pathogen, is a major cause of lower respiratory tract infections in infants and children. To investigate whether sncRNAs play a role in hMPV infection, we analyzed the changes in sncRNA profiles of airway epithelial cells in response to hMPV infection using ultrahigh-throughput sequencing. Of the cloned sncRNAs, miRNA was dominant in A549 cells, with the percentage of miRNA increasing in a time-dependent manner after the infection. In addition, several hMPV-derived sncRNAs and corresponding ribonucleases for their biogenesis were identified. hMPV M2-2 protein was revealed to be a key viral protein regulating miRNA expression. In summary, this study revealed several novel aspects of hMPV-mediated sncRNA expression, providing a new perspective on hMPV-host interactions.

AB - Small noncoding RNAs (sncRNAs), such as microRNAs (miRNA), virus-derived sncRNAs, and more recently identified tRNA-derived RNA fragments, are critical to posttranscriptional control of genes. Upon viral infection, host cells alter their sncRNA expression as a defense mechanism, while viruses can circumvent host defenses and promote their own propagation by affecting host cellular sncRNA expression or by expressing viral sncRNAs. Therefore, characterizing sncRNA profiles in response to viral infection is an important tool for understanding host-virus interaction, and for antiviral strategy development. Human metapneumovirus (hMPV), a recently identified pathogen, is a major cause of lower respiratory tract infections in infants and children. To investigate whether sncRNAs play a role in hMPV infection, we analyzed the changes in sncRNA profiles of airway epithelial cells in response to hMPV infection using ultrahigh-throughput sequencing. Of the cloned sncRNAs, miRNA was dominant in A549 cells, with the percentage of miRNA increasing in a time-dependent manner after the infection. In addition, several hMPV-derived sncRNAs and corresponding ribonucleases for their biogenesis were identified. hMPV M2-2 protein was revealed to be a key viral protein regulating miRNA expression. In summary, this study revealed several novel aspects of hMPV-mediated sncRNA expression, providing a new perspective on hMPV-host interactions.

KW - Human metapneumovirus

KW - Human metapneumovirus-derived snRNAs

KW - MiRNAs

KW - SncRNA

UR - http://www.scopus.com/inward/record.url?scp=84901237102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901237102&partnerID=8YFLogxK

U2 - 10.1038/mtna.2014.18

DO - 10.1038/mtna.2014.18

M3 - Article

VL - 3

JO - Molecular Therapy - Nucleic Acids

JF - Molecular Therapy - Nucleic Acids

SN - 2162-2531

M1 - e163

ER -