Abstract
The development of selective systems, in particular the HAT selective system, provided a crucial impetus in the development of somatic cell genetics. New selective systems should widen the scope of possible studies and be highly useful in the developing methods of gene transfer and regional mapping. Selective systems utilizing the purine and pyrimidine salvage pathways are particularly useful since both positive and negative systems can be devised for enzymes in these pathways, and the genes for a number of these enzymes are asyntenic. The method presented here involves positive and negative selection for adenosine kinase (ADK). The properties of some mouse human hybrids obtained with this selection is described.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 378-381 |
| Number of pages | 4 |
| Journal | Cytogenetics and Cell Genetics |
| Volume | 16 |
| Issue number | 1-5 |
| State | Published - 1976 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Genetics
Cite this
Human mouse somatic cell hybrid lines selected for human adenosine kinase : a new selective method. / Chan, T. S.; Creagan, R. P.
In: Cytogenetics and Cell Genetics, Vol. 16, No. 1-5, 1976, p. 378-381.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human mouse somatic cell hybrid lines selected for human adenosine kinase
T2 - a new selective method
AU - Chan, T. S.
AU - Creagan, R. P.
PY - 1976
Y1 - 1976
N2 - The development of selective systems, in particular the HAT selective system, provided a crucial impetus in the development of somatic cell genetics. New selective systems should widen the scope of possible studies and be highly useful in the developing methods of gene transfer and regional mapping. Selective systems utilizing the purine and pyrimidine salvage pathways are particularly useful since both positive and negative systems can be devised for enzymes in these pathways, and the genes for a number of these enzymes are asyntenic. The method presented here involves positive and negative selection for adenosine kinase (ADK). The properties of some mouse human hybrids obtained with this selection is described.
AB - The development of selective systems, in particular the HAT selective system, provided a crucial impetus in the development of somatic cell genetics. New selective systems should widen the scope of possible studies and be highly useful in the developing methods of gene transfer and regional mapping. Selective systems utilizing the purine and pyrimidine salvage pathways are particularly useful since both positive and negative systems can be devised for enzymes in these pathways, and the genes for a number of these enzymes are asyntenic. The method presented here involves positive and negative selection for adenosine kinase (ADK). The properties of some mouse human hybrids obtained with this selection is described.
UR - http://www.scopus.com/inward/record.url?scp=0017106922&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017106922&partnerID=8YFLogxK
M3 - Article
C2 - 185022
VL - 16
SP - 378
EP - 381
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
IS - 1-5
ER -