Human mouse somatic cell hybrid lines selected for human adenosine kinase: a new selective method

The development of selective systems, in particular the HAT selective system, provided a crucial impetus in the development of somatic cell genetics. New selective systems should widen the scope of possible studies and be highly useful in the developing methods of gene transfer and regional mapping. Selective systems utilizing the purine and pyrimidine salvage pathways are particularly useful since both positive and negative systems can be devised for enzymes in these pathways, and the genes for a number of these enzymes are asyntenic. The method presented here involves positive and negative selection for adenosine kinase (ADK). The properties of some mouse human hybrids obtained with this selection is described.