Human muscle gene expression following resistance exercise and blood flow restriction

Micah J. Drummond, Satoshi Fujita, Abe Takashi, Hans C. Dreyer, Elena Volpi, Blake Rasmussen

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

INTRODUCTION: Blood flow restriction in combination with low-intensity resistance exercise (REFR) increases skeletal muscle size to a similar extent as compared with traditional high-intensity resistance exercise training. However, there are limited data describing the molecular adaptations that occur after REFR. PURPOSE: To determine whether hypoxia inducible factor-1 alpha (HIF-1α) and REDD1 mRNA are expressed differently in REFR compared with low-intensity resistance exercise with no blood flow restriction (CONTROL). Secondly, to determine whether low-intensity resistance exercise is able to induce changes in mRNA expression of several anabolic and catabolic genes as typically seen with high-intensity resistance exercise. METHODS: Six subjects were studied at baseline and 3 h after a bout of leg resistance exercise (20% 1RM) in REFR and CONTROL subjects. Each subject participated in both groups, with 3 wk separating each visit. Muscle biopsy samples were analyzed for mRNA expression, using qRT-PCR. RESULTS: Our primary finding was that there were no differences between CONTROL and REFR for any of the selected genes at 3 h after exercise (P > 0.05). However, low-intensity resistance exercise increased HIF-1α, p21, MyoD, and muscle RING finger 1 (MuRF1) mRNA expression and decreased REDD1 and myostatin mRNA expression in both groups (P < 0.05). CONCLUSION: Low-intensity resistance exercise can alter skeletal muscle mRNA expression of several genes associated with muscle growth and remodeling, such as REDD1, HIF-1α, MyoD, MuRF1, and myostatin. Further, the results from REFR and CONTROL were similar, indicating that the changes in early postexercise gene expression were attributable to the low-intensity resistance exercise bout, and not blood flow restriction.

Original languageEnglish
Pages (from-to)691-698
Number of pages8
JournalMedicine and Science in Sports and Exercise
Volume40
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Gene Expression
Hypoxia-Inducible Factor 1
Muscles
Messenger RNA
Myostatin
Skeletal Muscle
Resistance Training
Genes
Leg
Exercise
Biopsy
Polymerase Chain Reaction
Growth

Keywords

  • HIF-1α
  • Ischemia-reperfusion
  • mRNA
  • mTOR
  • REDD1

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Cite this

Human muscle gene expression following resistance exercise and blood flow restriction. / Drummond, Micah J.; Fujita, Satoshi; Takashi, Abe; Dreyer, Hans C.; Volpi, Elena; Rasmussen, Blake.

In: Medicine and Science in Sports and Exercise, Vol. 40, No. 4, 04.2008, p. 691-698.

Research output: Contribution to journalArticle

Drummond, Micah J. ; Fujita, Satoshi ; Takashi, Abe ; Dreyer, Hans C. ; Volpi, Elena ; Rasmussen, Blake. / Human muscle gene expression following resistance exercise and blood flow restriction. In: Medicine and Science in Sports and Exercise. 2008 ; Vol. 40, No. 4. pp. 691-698.
@article{c262e06119ff41229f476f433e04cb19,
title = "Human muscle gene expression following resistance exercise and blood flow restriction",
abstract = "INTRODUCTION: Blood flow restriction in combination with low-intensity resistance exercise (REFR) increases skeletal muscle size to a similar extent as compared with traditional high-intensity resistance exercise training. However, there are limited data describing the molecular adaptations that occur after REFR. PURPOSE: To determine whether hypoxia inducible factor-1 alpha (HIF-1α) and REDD1 mRNA are expressed differently in REFR compared with low-intensity resistance exercise with no blood flow restriction (CONTROL). Secondly, to determine whether low-intensity resistance exercise is able to induce changes in mRNA expression of several anabolic and catabolic genes as typically seen with high-intensity resistance exercise. METHODS: Six subjects were studied at baseline and 3 h after a bout of leg resistance exercise (20{\%} 1RM) in REFR and CONTROL subjects. Each subject participated in both groups, with 3 wk separating each visit. Muscle biopsy samples were analyzed for mRNA expression, using qRT-PCR. RESULTS: Our primary finding was that there were no differences between CONTROL and REFR for any of the selected genes at 3 h after exercise (P > 0.05). However, low-intensity resistance exercise increased HIF-1α, p21, MyoD, and muscle RING finger 1 (MuRF1) mRNA expression and decreased REDD1 and myostatin mRNA expression in both groups (P < 0.05). CONCLUSION: Low-intensity resistance exercise can alter skeletal muscle mRNA expression of several genes associated with muscle growth and remodeling, such as REDD1, HIF-1α, MyoD, MuRF1, and myostatin. Further, the results from REFR and CONTROL were similar, indicating that the changes in early postexercise gene expression were attributable to the low-intensity resistance exercise bout, and not blood flow restriction.",
keywords = "HIF-1α, Ischemia-reperfusion, mRNA, mTOR, REDD1",
author = "Drummond, {Micah J.} and Satoshi Fujita and Abe Takashi and Dreyer, {Hans C.} and Elena Volpi and Blake Rasmussen",
year = "2008",
month = "4",
doi = "10.1249/MSS.0b013e318160ff84",
language = "English",
volume = "40",
pages = "691--698",
journal = "Medicine and Science in Sports and Exercise",
issn = "0195-9131",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Human muscle gene expression following resistance exercise and blood flow restriction

AU - Drummond, Micah J.

AU - Fujita, Satoshi

AU - Takashi, Abe

AU - Dreyer, Hans C.

AU - Volpi, Elena

AU - Rasmussen, Blake

PY - 2008/4

Y1 - 2008/4

N2 - INTRODUCTION: Blood flow restriction in combination with low-intensity resistance exercise (REFR) increases skeletal muscle size to a similar extent as compared with traditional high-intensity resistance exercise training. However, there are limited data describing the molecular adaptations that occur after REFR. PURPOSE: To determine whether hypoxia inducible factor-1 alpha (HIF-1α) and REDD1 mRNA are expressed differently in REFR compared with low-intensity resistance exercise with no blood flow restriction (CONTROL). Secondly, to determine whether low-intensity resistance exercise is able to induce changes in mRNA expression of several anabolic and catabolic genes as typically seen with high-intensity resistance exercise. METHODS: Six subjects were studied at baseline and 3 h after a bout of leg resistance exercise (20% 1RM) in REFR and CONTROL subjects. Each subject participated in both groups, with 3 wk separating each visit. Muscle biopsy samples were analyzed for mRNA expression, using qRT-PCR. RESULTS: Our primary finding was that there were no differences between CONTROL and REFR for any of the selected genes at 3 h after exercise (P > 0.05). However, low-intensity resistance exercise increased HIF-1α, p21, MyoD, and muscle RING finger 1 (MuRF1) mRNA expression and decreased REDD1 and myostatin mRNA expression in both groups (P < 0.05). CONCLUSION: Low-intensity resistance exercise can alter skeletal muscle mRNA expression of several genes associated with muscle growth and remodeling, such as REDD1, HIF-1α, MyoD, MuRF1, and myostatin. Further, the results from REFR and CONTROL were similar, indicating that the changes in early postexercise gene expression were attributable to the low-intensity resistance exercise bout, and not blood flow restriction.

AB - INTRODUCTION: Blood flow restriction in combination with low-intensity resistance exercise (REFR) increases skeletal muscle size to a similar extent as compared with traditional high-intensity resistance exercise training. However, there are limited data describing the molecular adaptations that occur after REFR. PURPOSE: To determine whether hypoxia inducible factor-1 alpha (HIF-1α) and REDD1 mRNA are expressed differently in REFR compared with low-intensity resistance exercise with no blood flow restriction (CONTROL). Secondly, to determine whether low-intensity resistance exercise is able to induce changes in mRNA expression of several anabolic and catabolic genes as typically seen with high-intensity resistance exercise. METHODS: Six subjects were studied at baseline and 3 h after a bout of leg resistance exercise (20% 1RM) in REFR and CONTROL subjects. Each subject participated in both groups, with 3 wk separating each visit. Muscle biopsy samples were analyzed for mRNA expression, using qRT-PCR. RESULTS: Our primary finding was that there were no differences between CONTROL and REFR for any of the selected genes at 3 h after exercise (P > 0.05). However, low-intensity resistance exercise increased HIF-1α, p21, MyoD, and muscle RING finger 1 (MuRF1) mRNA expression and decreased REDD1 and myostatin mRNA expression in both groups (P < 0.05). CONCLUSION: Low-intensity resistance exercise can alter skeletal muscle mRNA expression of several genes associated with muscle growth and remodeling, such as REDD1, HIF-1α, MyoD, MuRF1, and myostatin. Further, the results from REFR and CONTROL were similar, indicating that the changes in early postexercise gene expression were attributable to the low-intensity resistance exercise bout, and not blood flow restriction.

KW - HIF-1α

KW - Ischemia-reperfusion

KW - mRNA

KW - mTOR

KW - REDD1

UR - http://www.scopus.com/inward/record.url?scp=40949095311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40949095311&partnerID=8YFLogxK

U2 - 10.1249/MSS.0b013e318160ff84

DO - 10.1249/MSS.0b013e318160ff84

M3 - Article

VL - 40

SP - 691

EP - 698

JO - Medicine and Science in Sports and Exercise

JF - Medicine and Science in Sports and Exercise

SN - 0195-9131

IS - 4

ER -