Human serum immunoglobulin counteracts rotaviral infection in Caco-2 cells

Alfredo Guarino, Antonella Casola, Eugenia Bruzzese, Marco Saini, Lucio Nitsch, Armido Rubino

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Oral administration of human serum immunoglobulin reduces the duration of diarrhea and of rotaviral excretion in children. To investigate the in vitro effects of immunoglobulin on virus-enterocyte interaction, Caco-2 cells were infected with Rotavirus strain SA11. Immunoglobulin was added prior to and at various times postinfection. Indirect immunofluorescence was performed with an antibody against VP-6 rotaviral antigen. Cell viability and monolayer transepithelial electrical resistance (TEER) were monitored. Immunofluorescence showed a perinuclear distribution in 90% of cells. Rotavirus infection induced a progressive decrease in TEER and a parallel reduction in cell viability, depending on viral load. Preincubation of the virus with immunoglobulin prevented cell infection as judged by immunofluorescence. Immunoglobulin addition to infected cells partially prevented the decrease in TEER and induced a later shift of TEER toward increasing values, suggesting restoration of monolayer's integrity. The efficacy of immunoglobulin depended on its concentration and on the time of its addition. These results indicate that immunoglobulin is effective in preventing infection and in reducing cell damage, through a direct anti- Rotavirus action and may indicate that immunoglobulin should be administered in the early phase of diarrhea, to reduce the severity of Rotavirus infection.

Original languageEnglish (US)
Pages (from-to)881-887
Number of pages7
JournalPediatric Research
Volume40
Issue number6
StatePublished - Dec 1996
Externally publishedYes

Fingerprint

Caco-2 Cells
Immunoglobulins
Infection
Serum
Electric Impedance
Rotavirus Infections
Rotavirus
Fluorescent Antibody Technique
Diarrhea
Cell Survival
Viruses
Viral Structural Proteins
Enterocytes
Indirect Fluorescent Antibody Technique
Viral Load
Oral Administration
Antigens
Antibodies

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Guarino, A., Casola, A., Bruzzese, E., Saini, M., Nitsch, L., & Rubino, A. (1996). Human serum immunoglobulin counteracts rotaviral infection in Caco-2 cells. Pediatric Research, 40(6), 881-887.

Human serum immunoglobulin counteracts rotaviral infection in Caco-2 cells. / Guarino, Alfredo; Casola, Antonella; Bruzzese, Eugenia; Saini, Marco; Nitsch, Lucio; Rubino, Armido.

In: Pediatric Research, Vol. 40, No. 6, 12.1996, p. 881-887.

Research output: Contribution to journalArticle

Guarino, A, Casola, A, Bruzzese, E, Saini, M, Nitsch, L & Rubino, A 1996, 'Human serum immunoglobulin counteracts rotaviral infection in Caco-2 cells', Pediatric Research, vol. 40, no. 6, pp. 881-887.
Guarino A, Casola A, Bruzzese E, Saini M, Nitsch L, Rubino A. Human serum immunoglobulin counteracts rotaviral infection in Caco-2 cells. Pediatric Research. 1996 Dec;40(6):881-887.
Guarino, Alfredo ; Casola, Antonella ; Bruzzese, Eugenia ; Saini, Marco ; Nitsch, Lucio ; Rubino, Armido. / Human serum immunoglobulin counteracts rotaviral infection in Caco-2 cells. In: Pediatric Research. 1996 ; Vol. 40, No. 6. pp. 881-887.
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