Human skeletal muscle macrophages increase following cycle training and are associated with adaptations that may facilitate growth

R. Grace Walton, Kate Kosmac, Jyothi Mula, Christopher Fry, Bailey D. Peck, Jason S. Groshong, Brian S. Finlin, Beibei Zhu, Philip A. Kern, Charlotte A. Peterson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Skeletal muscle macrophages participate in repair and regeneration following injury. However, their role in physiological adaptations to exercise is unexplored. We determined whether endurance exercise training (EET) alters macrophage content and characteristics in response to resistance exercise (RE), and whether macrophages are associated with other exercise adaptations. Subjects provided vastus lateralis biopsies before and after one bout of RE, after 12 weeks of EET (cycling), and after a final bout of RE. M2 macrophages (CD11b+/CD206+) did not increase with RE, but increased in response to EET (P < 0.01). Increases in M2 macrophages were positively correlated with fiber hypertrophy (r = 0.49) and satellite cells (r = 0.47). M2c macrophages (CD206+/CD163+) also increased following EET (P < 0.001), and were associated with fiber hypertrophy (r = 0.64). Gene expression was quantified using NanoString. Following EET, the change in M2 macrophages was positively associated with changes in HGF, IGF1, and extracellular matrix genes. EET decreased expression of IL6 (P < 0.05), C/EBPβ (P < 0.01), and MuRF (P < 0.05), and increased expression of IL-4 (P < 0.01), TNFα (P < 0.01) and the TWEAK receptor FN14 (P < 0.05). The change in FN14 gene expression was inversely associated with changes in C/EBPβ (r = −0.58) and MuRF (r = −0.46) following EET. In cultured human myotubes, siRNA inhibition of FN14 increased expression of C/EBPβ (P < 0.05) and MuRF (P < 0.05). Our data suggest that macrophages contribute to the muscle response to EET, potentially including modulation of TWEAK-FN14 signaling.

Original languageEnglish (US)
Article number969
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Skeletal Muscle
Macrophages
Exercise
Growth
Hypertrophy
Physiological Adaptation
Gene Expression
Skeletal Muscle Fibers
Quadriceps Muscle
Interleukin-4
Small Interfering RNA
Extracellular Matrix
Regeneration
Interleukin-6
Biopsy
Muscles
Wounds and Injuries
Genes

ASJC Scopus subject areas

  • General

Cite this

Human skeletal muscle macrophages increase following cycle training and are associated with adaptations that may facilitate growth. / Walton, R. Grace; Kosmac, Kate; Mula, Jyothi; Fry, Christopher; Peck, Bailey D.; Groshong, Jason S.; Finlin, Brian S.; Zhu, Beibei; Kern, Philip A.; Peterson, Charlotte A.

In: Scientific Reports, Vol. 9, No. 1, 969, 01.12.2019.

Research output: Contribution to journalArticle

Walton, RG, Kosmac, K, Mula, J, Fry, C, Peck, BD, Groshong, JS, Finlin, BS, Zhu, B, Kern, PA & Peterson, CA 2019, 'Human skeletal muscle macrophages increase following cycle training and are associated with adaptations that may facilitate growth', Scientific Reports, vol. 9, no. 1, 969. https://doi.org/10.1038/s41598-018-37187-1
Walton, R. Grace ; Kosmac, Kate ; Mula, Jyothi ; Fry, Christopher ; Peck, Bailey D. ; Groshong, Jason S. ; Finlin, Brian S. ; Zhu, Beibei ; Kern, Philip A. ; Peterson, Charlotte A. / Human skeletal muscle macrophages increase following cycle training and are associated with adaptations that may facilitate growth. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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