Human sperm bioassay for reprotoxicity testing in embryo culture media: Some practical considerations in reducing the assay time

Amjad Hossain, Subhash Aryal, Collin Osuampke, John Phelps

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture media, we compared American-Association-of-Bioanalysts-(AAB-) administered HSA data to our own assay performed using PT samples obtained from AAB. Participating laboratories were required to culture sperm for 48 hours to determine media acceptability. Conclusions drawn from 48- and 24-hour observations were the same, suggesting that HSA could identify reprotoxic media in less time than required by AAB. Our assay revealed that changes in motility grade in adulterated media are significantly different from those in control media. Furthermore, grade changes can be identified earlier than differences in motility loss between samples. Analyzing motility and motility quality together provides a method for establishing an optimal time for HSA.

Original languageEnglish (US)
Article number136898
JournalAdvances in Urology
DOIs
StatePublished - 2010

Fingerprint

Biological Assay
Culture Media
Spermatozoa
Embryonic Structures
Quality Control
Fertility

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Urology

Cite this

Human sperm bioassay for reprotoxicity testing in embryo culture media : Some practical considerations in reducing the assay time. / Hossain, Amjad; Aryal, Subhash; Osuampke, Collin; Phelps, John.

In: Advances in Urology, 2010.

Research output: Contribution to journalArticle

@article{d1339617add1459b880ae69720549201,
title = "Human sperm bioassay for reprotoxicity testing in embryo culture media: Some practical considerations in reducing the assay time",
abstract = "Human sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture media, we compared American-Association-of-Bioanalysts-(AAB-) administered HSA data to our own assay performed using PT samples obtained from AAB. Participating laboratories were required to culture sperm for 48 hours to determine media acceptability. Conclusions drawn from 48- and 24-hour observations were the same, suggesting that HSA could identify reprotoxic media in less time than required by AAB. Our assay revealed that changes in motility grade in adulterated media are significantly different from those in control media. Furthermore, grade changes can be identified earlier than differences in motility loss between samples. Analyzing motility and motility quality together provides a method for establishing an optimal time for HSA.",
author = "Amjad Hossain and Subhash Aryal and Collin Osuampke and John Phelps",
year = "2010",
doi = "10.1155/2010/136898",
language = "English (US)",
journal = "Advances in Urology",
issn = "1687-6369",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Human sperm bioassay for reprotoxicity testing in embryo culture media

T2 - Some practical considerations in reducing the assay time

AU - Hossain, Amjad

AU - Aryal, Subhash

AU - Osuampke, Collin

AU - Phelps, John

PY - 2010

Y1 - 2010

N2 - Human sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture media, we compared American-Association-of-Bioanalysts-(AAB-) administered HSA data to our own assay performed using PT samples obtained from AAB. Participating laboratories were required to culture sperm for 48 hours to determine media acceptability. Conclusions drawn from 48- and 24-hour observations were the same, suggesting that HSA could identify reprotoxic media in less time than required by AAB. Our assay revealed that changes in motility grade in adulterated media are significantly different from those in control media. Furthermore, grade changes can be identified earlier than differences in motility loss between samples. Analyzing motility and motility quality together provides a method for establishing an optimal time for HSA.

AB - Human sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture media, we compared American-Association-of-Bioanalysts-(AAB-) administered HSA data to our own assay performed using PT samples obtained from AAB. Participating laboratories were required to culture sperm for 48 hours to determine media acceptability. Conclusions drawn from 48- and 24-hour observations were the same, suggesting that HSA could identify reprotoxic media in less time than required by AAB. Our assay revealed that changes in motility grade in adulterated media are significantly different from those in control media. Furthermore, grade changes can be identified earlier than differences in motility loss between samples. Analyzing motility and motility quality together provides a method for establishing an optimal time for HSA.

UR - http://www.scopus.com/inward/record.url?scp=79251552280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251552280&partnerID=8YFLogxK

U2 - 10.1155/2010/136898

DO - 10.1155/2010/136898

M3 - Article

C2 - 21234335

AN - SCOPUS:79251552280

JO - Advances in Urology

JF - Advances in Urology

SN - 1687-6369

M1 - 136898

ER -