TY - JOUR
T1 - Human sperm bioassay has potential in evaluating the quality of cumulus-oocyte complexes
AU - Hossain, A. M.
AU - Rizk, B.
AU - Huff, C.
AU - Helvacioglu, A.
AU - Thorneycroft, I. H.
PY - 1996
Y1 - 1996
N2 - Human sperm bioassay is routinely used as a quality control check for the culture media. This is one of the three bioassays chosen by the College of American Pathologists (CAP) for interlaboratory proficiency testing to assess the standards of in vitro fertilization (IVF) and andrology laboratories. This study utilized sperm bioassay to assess the quality of cumulus-oocyte complexes (COCs) retrieved in IVF procedures. COCs, harvested from the female partner of IVF couples, undergoing identical ovarian stimulation protocols, were individually inseminated with the sperm of the corresponding male partner. Sperm motility in sperm-COG cocultures were compared. Cocultures were established by inseminating the 103 COCs, retrieved from 18 IVF couples, with 1 x 105 to 2 x 105 sperm of the corresponding male partners of the couples. In all 18 cases, the sperm were prepared identically using the Percoll wash method. The cocultures were maintained for 48 h but the oocytes were removed immediately after the fertilization check (approximately 16 h). The motility of sperm in the cocultures and in the insemination stocks were noted and 17 of 18 sperm stocks used for insemination had similar high preinsemination motility (90.2 ± 5.0%). At 48 h the sperm, motility had significantly decreased in the cocultures compared to the insemination stocks; 52.7 ± 19.9% versus 67.2 ± 10.4%. There was no difference in the motility among the small, medium, and large COCs (56.4 ± 24.6%, 52.5 ± 17.9%, and 50.8 ± 20.9%, respectively). In 45% of IVF cases, the motility in cocultures varied widely, falling below as well as above that of their corresponding insemination stocks. Furthermore, the sperm motility varied among the cocultures in both pregnant and nonpregnant patients but the extent of variation appears to be greater in the latter. The inter-COG coculture sperm motility variation most likely is due to the differences in the quality of cumulus-oocyte complexes.
AB - Human sperm bioassay is routinely used as a quality control check for the culture media. This is one of the three bioassays chosen by the College of American Pathologists (CAP) for interlaboratory proficiency testing to assess the standards of in vitro fertilization (IVF) and andrology laboratories. This study utilized sperm bioassay to assess the quality of cumulus-oocyte complexes (COCs) retrieved in IVF procedures. COCs, harvested from the female partner of IVF couples, undergoing identical ovarian stimulation protocols, were individually inseminated with the sperm of the corresponding male partner. Sperm motility in sperm-COG cocultures were compared. Cocultures were established by inseminating the 103 COCs, retrieved from 18 IVF couples, with 1 x 105 to 2 x 105 sperm of the corresponding male partners of the couples. In all 18 cases, the sperm were prepared identically using the Percoll wash method. The cocultures were maintained for 48 h but the oocytes were removed immediately after the fertilization check (approximately 16 h). The motility of sperm in the cocultures and in the insemination stocks were noted and 17 of 18 sperm stocks used for insemination had similar high preinsemination motility (90.2 ± 5.0%). At 48 h the sperm, motility had significantly decreased in the cocultures compared to the insemination stocks; 52.7 ± 19.9% versus 67.2 ± 10.4%. There was no difference in the motility among the small, medium, and large COCs (56.4 ± 24.6%, 52.5 ± 17.9%, and 50.8 ± 20.9%, respectively). In 45% of IVF cases, the motility in cocultures varied widely, falling below as well as above that of their corresponding insemination stocks. Furthermore, the sperm motility varied among the cocultures in both pregnant and nonpregnant patients but the extent of variation appears to be greater in the latter. The inter-COG coculture sperm motility variation most likely is due to the differences in the quality of cumulus-oocyte complexes.
KW - Coculture
KW - Corona
KW - Cumulus cells
KW - IVF
KW - Sperm bioassay
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UR - http://www.scopus.com/inward/citedby.url?scp=79961231258&partnerID=8YFLogxK
U2 - 10.3109/01485019608988495
DO - 10.3109/01485019608988495
M3 - Article
C2 - 8827341
AN - SCOPUS:79961231258
SN - 0148-5016
VL - 37
SP - 7
EP - 10
JO - Archives of Andrology
JF - Archives of Andrology
IS - 1
ER -