Human T-cell recognition of Listeria monocytogenes: Recognition of listeriolysin O by TcRαβ+ and TcRγδ+ T cells

Y. Guo, H. K. Ziegler, S. A. Safley, David Niesel, S. Vaidya, G. R. Klimpel

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The cell-mediated immune response to Listeria monocytogenes has been well characterized in the mouse. Listeriolysin O (LLO) is a major antigen in marine T-cell recognition of L. monocytogenes. In this study, we show that LLO is also recognized by human TcRαβ T cells and TcRγδ T cells. Human peripheral blood mononuclear cells (PBMC) cultured in vitro with live listeriae and then expanded with interleukin 2 were shown to respond to purified LLO. The generation of LLO-responsive T cells was dependent on the use of live bacteria during the initial in vitro challenge. LLO-induced proliferation of T cells expanded by exposure of PBMC to live listeriae was major histocompatibility complex restricted. PBMC cultured with formalin- fixed listeriae and subsequently expanded by interleukin 2 gave high proliferative responses to fixed bacteria but failed to respond to LLO. PBMC stimulated in vitro with fixed listeriae contained predominantly TcRαβ+ T cells. In contrast, PBMC obtained from 85% of the donors studied generated high numbers of TcRγδ+ T cells following in vitro culture with live listeriae. Using a panel of synthetic amphipathic LLO peptides, we found that LLO-specific T cells from different individuals recognized both common and unique peptides. LLO 470-508 was recognized by three of five individuals, while LLO 203-226 and LLO 107-126 were recognized by two of six individuals. A TcRγδ+ T-cell line was established from PBMC stimulated with live listeriae and was shown to recognize LLO 470-508. Proliferative responses could be induced in this cell line by peptide-pulsed autologous PBMC but not by peptide-pulsed allogeneic PBMC. Our results establish the importance of LLO in human T-cell recognition of listeriae and show that both TcRαβ+ T cells and TcRγδ+ T cells recognize this antigen. Finally, since LLO 470- 508 has a high degree of homology with other gram-positive bacterial toxins, the recognition of this peptide by TcRγδ+ T cells suggests that an important role of these T cells in host defense is the recognition of bacterium-derived toxins.

Original languageEnglish (US)
Pages (from-to)2288-2294
Number of pages7
JournalInfection and Immunity
Volume63
Issue number6
StatePublished - 1995

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Listeria monocytogenes
T-Lymphocytes
Listeria
Blood Cells
Peptides
Listeria monocytogenes hlyA protein
Bacteria
Interleukin-2
Peptide T
Bacterial Toxins
Antigens
Cell Line
Major Histocompatibility Complex
Formaldehyde

ASJC Scopus subject areas

  • Immunology

Cite this

Human T-cell recognition of Listeria monocytogenes : Recognition of listeriolysin O by TcRαβ+ and TcRγδ+ T cells. / Guo, Y.; Ziegler, H. K.; Safley, S. A.; Niesel, David; Vaidya, S.; Klimpel, G. R.

In: Infection and Immunity, Vol. 63, No. 6, 1995, p. 2288-2294.

Research output: Contribution to journalArticle

Guo, Y, Ziegler, HK, Safley, SA, Niesel, D, Vaidya, S & Klimpel, GR 1995, 'Human T-cell recognition of Listeria monocytogenes: Recognition of listeriolysin O by TcRαβ+ and TcRγδ+ T cells', Infection and Immunity, vol. 63, no. 6, pp. 2288-2294.
Guo, Y. ; Ziegler, H. K. ; Safley, S. A. ; Niesel, David ; Vaidya, S. ; Klimpel, G. R. / Human T-cell recognition of Listeria monocytogenes : Recognition of listeriolysin O by TcRαβ+ and TcRγδ+ T cells. In: Infection and Immunity. 1995 ; Vol. 63, No. 6. pp. 2288-2294.
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abstract = "The cell-mediated immune response to Listeria monocytogenes has been well characterized in the mouse. Listeriolysin O (LLO) is a major antigen in marine T-cell recognition of L. monocytogenes. In this study, we show that LLO is also recognized by human TcRαβ T cells and TcRγδ T cells. Human peripheral blood mononuclear cells (PBMC) cultured in vitro with live listeriae and then expanded with interleukin 2 were shown to respond to purified LLO. The generation of LLO-responsive T cells was dependent on the use of live bacteria during the initial in vitro challenge. LLO-induced proliferation of T cells expanded by exposure of PBMC to live listeriae was major histocompatibility complex restricted. PBMC cultured with formalin- fixed listeriae and subsequently expanded by interleukin 2 gave high proliferative responses to fixed bacteria but failed to respond to LLO. PBMC stimulated in vitro with fixed listeriae contained predominantly TcRαβ+ T cells. In contrast, PBMC obtained from 85{\%} of the donors studied generated high numbers of TcRγδ+ T cells following in vitro culture with live listeriae. Using a panel of synthetic amphipathic LLO peptides, we found that LLO-specific T cells from different individuals recognized both common and unique peptides. LLO 470-508 was recognized by three of five individuals, while LLO 203-226 and LLO 107-126 were recognized by two of six individuals. A TcRγδ+ T-cell line was established from PBMC stimulated with live listeriae and was shown to recognize LLO 470-508. Proliferative responses could be induced in this cell line by peptide-pulsed autologous PBMC but not by peptide-pulsed allogeneic PBMC. Our results establish the importance of LLO in human T-cell recognition of listeriae and show that both TcRαβ+ T cells and TcRγδ+ T cells recognize this antigen. Finally, since LLO 470- 508 has a high degree of homology with other gram-positive bacterial toxins, the recognition of this peptide by TcRγδ+ T cells suggests that an important role of these T cells in host defense is the recognition of bacterium-derived toxins.",
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T2 - Recognition of listeriolysin O by TcRαβ+ and TcRγδ+ T cells

AU - Guo, Y.

AU - Ziegler, H. K.

AU - Safley, S. A.

AU - Niesel, David

AU - Vaidya, S.

AU - Klimpel, G. R.

PY - 1995

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N2 - The cell-mediated immune response to Listeria monocytogenes has been well characterized in the mouse. Listeriolysin O (LLO) is a major antigen in marine T-cell recognition of L. monocytogenes. In this study, we show that LLO is also recognized by human TcRαβ T cells and TcRγδ T cells. Human peripheral blood mononuclear cells (PBMC) cultured in vitro with live listeriae and then expanded with interleukin 2 were shown to respond to purified LLO. The generation of LLO-responsive T cells was dependent on the use of live bacteria during the initial in vitro challenge. LLO-induced proliferation of T cells expanded by exposure of PBMC to live listeriae was major histocompatibility complex restricted. PBMC cultured with formalin- fixed listeriae and subsequently expanded by interleukin 2 gave high proliferative responses to fixed bacteria but failed to respond to LLO. PBMC stimulated in vitro with fixed listeriae contained predominantly TcRαβ+ T cells. In contrast, PBMC obtained from 85% of the donors studied generated high numbers of TcRγδ+ T cells following in vitro culture with live listeriae. Using a panel of synthetic amphipathic LLO peptides, we found that LLO-specific T cells from different individuals recognized both common and unique peptides. LLO 470-508 was recognized by three of five individuals, while LLO 203-226 and LLO 107-126 were recognized by two of six individuals. A TcRγδ+ T-cell line was established from PBMC stimulated with live listeriae and was shown to recognize LLO 470-508. Proliferative responses could be induced in this cell line by peptide-pulsed autologous PBMC but not by peptide-pulsed allogeneic PBMC. Our results establish the importance of LLO in human T-cell recognition of listeriae and show that both TcRαβ+ T cells and TcRγδ+ T cells recognize this antigen. Finally, since LLO 470- 508 has a high degree of homology with other gram-positive bacterial toxins, the recognition of this peptide by TcRγδ+ T cells suggests that an important role of these T cells in host defense is the recognition of bacterium-derived toxins.

AB - The cell-mediated immune response to Listeria monocytogenes has been well characterized in the mouse. Listeriolysin O (LLO) is a major antigen in marine T-cell recognition of L. monocytogenes. In this study, we show that LLO is also recognized by human TcRαβ T cells and TcRγδ T cells. Human peripheral blood mononuclear cells (PBMC) cultured in vitro with live listeriae and then expanded with interleukin 2 were shown to respond to purified LLO. The generation of LLO-responsive T cells was dependent on the use of live bacteria during the initial in vitro challenge. LLO-induced proliferation of T cells expanded by exposure of PBMC to live listeriae was major histocompatibility complex restricted. PBMC cultured with formalin- fixed listeriae and subsequently expanded by interleukin 2 gave high proliferative responses to fixed bacteria but failed to respond to LLO. PBMC stimulated in vitro with fixed listeriae contained predominantly TcRαβ+ T cells. In contrast, PBMC obtained from 85% of the donors studied generated high numbers of TcRγδ+ T cells following in vitro culture with live listeriae. Using a panel of synthetic amphipathic LLO peptides, we found that LLO-specific T cells from different individuals recognized both common and unique peptides. LLO 470-508 was recognized by three of five individuals, while LLO 203-226 and LLO 107-126 were recognized by two of six individuals. A TcRγδ+ T-cell line was established from PBMC stimulated with live listeriae and was shown to recognize LLO 470-508. Proliferative responses could be induced in this cell line by peptide-pulsed autologous PBMC but not by peptide-pulsed allogeneic PBMC. Our results establish the importance of LLO in human T-cell recognition of listeriae and show that both TcRαβ+ T cells and TcRγδ+ T cells recognize this antigen. Finally, since LLO 470- 508 has a high degree of homology with other gram-positive bacterial toxins, the recognition of this peptide by TcRγδ+ T cells suggests that an important role of these T cells in host defense is the recognition of bacterium-derived toxins.

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