Human xeroderma pigmentosum group g gene encodes a DNA endonuclease

Yvette Habraken, Patrick Sung, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Because of defective nucleotide excision repair of ultraviolet damaged DNA, xeroderma pigmentosum (XP) patients suffer from a high incidence of skin cancers. Cell fusion studies have identified seven XP complementation groups, A to G. Previous studies have implicated the products of these seven XP genes in the recognition of ultraviolet-induced DNA damage and in incision of the damage-containing DNA strand. Here, we express the XPG-encoded protein in Sf9 insect cells and purify it to homogeneity. We demonstrate that XPG is a single-strand specific DNA endonuclease, thus identifying the catalytic role of the protein in nucleotide excision repair. We suggest that XPG nuclease acts on the single-stranded region created as a result of the combined action of the XPB helicase and XPD helicase at the DNA damage site.

Original languageEnglish
Pages (from-to)3312-3316
Number of pages5
JournalNucleic Acids Research
Volume22
Issue number16
StatePublished - Aug 25 1994

Fingerprint

Xeroderma Pigmentosum
Deoxyribonuclease I
DNA Damage
DNA
Damage
Genes
Gene
Ultraviolet
DNA Repair
Repair
Protein
Sf9 Cells
Nucleotides
Complementation
Cell Fusion
Cell
Skin Neoplasms
Homogeneity
Skin
Insects

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Human xeroderma pigmentosum group g gene encodes a DNA endonuclease. / Habraken, Yvette; Sung, Patrick; Prakash, Louise; Prakash, Satya.

In: Nucleic Acids Research, Vol. 22, No. 16, 25.08.1994, p. 3312-3316.

Research output: Contribution to journalArticle

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