Humoral and cellular immune responses to Yersinia pestis Pla antigen in humans immunized with live plague vaccine

Valentina A. Feodorova, Anna M. Lyapina, Maria A. Khizhnyakova, Sergey S. Zaitsev, Lidiya V. Sayapina, Tatiana E. Arseneva, Alexey L. Trukhachev, Svetlana A. Lebedeva, Maxim V. Telepnev, Onega V. Ulianova, Elena P. Lyapina, Sergey S. Ulyanov, Vladimir Motin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: To establish correlates of human immunity to the live plague vaccine (LPV), we analyzed parameters of cellular and antibody response to the plasminogen activator Pla of Y. pestis. This outer membrane protease is an essential virulence factor that is steadily expressed by Y. pestis. Methodology/Principal findings: PBMCs and sera were obtained from a cohort of naïve (n = 17) and LPV-vaccinated (n = 34) donors. Anti-Pla antibodies of different classes and IgG subclasses were determined by ELISA and immunoblotting. The analysis of antibody response was complicated with a strong reactivity of Pla with normal human sera. The linear Pla B-cell epitopes were mapped using a library of 15-mer overlapping peptides. Twelve peptides that reacted specifically with sera of vaccinated donors were found together with a major cross-reacting peptide IPNISPDSFTVAAST located at the N-terminus. PBMCs were stimulated with recombinant Pla followed by proliferative analysis and cytokine profiling. The T-cell recall response was pronounced in vaccinees less than a year post-immunization, and became Th17-polarized over time after many rounds of vaccination. Conclusions/Significance: The Pla protein can serve as a biomarker of successful vaccination with LPV. The diagnostic use of Pla will require elimination of cross-reactive parts of the antigen.

Original languageEnglish (US)
Article numbere0006511
JournalPLoS Neglected Tropical Diseases
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2018

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Plague Vaccine
Yersinia pestis
Humoral Immunity
Cellular Immunity
Antigens
Peptides
Antibody Formation
Vaccination
Serum
B-Lymphocyte Epitopes
Immunoglobulin Isotypes
Plasminogen Activators
Virulence Factors
Immunoblotting
Libraries
Anti-Idiotypic Antibodies
Immunity
Immunization
Peptide Hydrolases
Immunoglobulin G

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Humoral and cellular immune responses to Yersinia pestis Pla antigen in humans immunized with live plague vaccine. / Feodorova, Valentina A.; Lyapina, Anna M.; Khizhnyakova, Maria A.; Zaitsev, Sergey S.; Sayapina, Lidiya V.; Arseneva, Tatiana E.; Trukhachev, Alexey L.; Lebedeva, Svetlana A.; Telepnev, Maxim V.; Ulianova, Onega V.; Lyapina, Elena P.; Ulyanov, Sergey S.; Motin, Vladimir.

In: PLoS Neglected Tropical Diseases, Vol. 12, No. 6, e0006511, 01.06.2018.

Research output: Contribution to journalArticle

Feodorova, VA, Lyapina, AM, Khizhnyakova, MA, Zaitsev, SS, Sayapina, LV, Arseneva, TE, Trukhachev, AL, Lebedeva, SA, Telepnev, MV, Ulianova, OV, Lyapina, EP, Ulyanov, SS & Motin, V 2018, 'Humoral and cellular immune responses to Yersinia pestis Pla antigen in humans immunized with live plague vaccine', PLoS Neglected Tropical Diseases, vol. 12, no. 6, e0006511. https://doi.org/10.1371/journal.pntd.0006511
Feodorova, Valentina A. ; Lyapina, Anna M. ; Khizhnyakova, Maria A. ; Zaitsev, Sergey S. ; Sayapina, Lidiya V. ; Arseneva, Tatiana E. ; Trukhachev, Alexey L. ; Lebedeva, Svetlana A. ; Telepnev, Maxim V. ; Ulianova, Onega V. ; Lyapina, Elena P. ; Ulyanov, Sergey S. ; Motin, Vladimir. / Humoral and cellular immune responses to Yersinia pestis Pla antigen in humans immunized with live plague vaccine. In: PLoS Neglected Tropical Diseases. 2018 ; Vol. 12, No. 6.
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AU - Zaitsev, Sergey S.

AU - Sayapina, Lidiya V.

AU - Arseneva, Tatiana E.

AU - Trukhachev, Alexey L.

AU - Lebedeva, Svetlana A.

AU - Telepnev, Maxim V.

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AU - Lyapina, Elena P.

AU - Ulyanov, Sergey S.

AU - Motin, Vladimir

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