Hydralazine causes nonspecific binding of antibodies to human lymphocytes in vitro

Eugene J. Kucharz, James Goodwin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In vitro incubation of human lymphocytes or a continuous human Jurkat T cell line with hydralazine (1-hydrazinophthalazine), a widely used antihypertensive agent, produced a nonspecific binding of various antibodies to the cells. This phenomenon was found to produce increases in staining of mitogen-stimulated and mitogen-unstimulated human lymphocytes and Jurkat cells with a variety of antibodies recognizing T and B cell antigens. The increase in nonspecific binding was greatest at 10-2 m hydralazine, but was seen with concentrations as low 10-7 M, which is comparable to the serum concentrations of patients prescribed hydralazine. The binding of antibodies is not connected with significant decreases in the cell viability. This property of hydralazine may cause misinterpretation of certain immunological tests employing the antibodies.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalImmunopharmacology
Volume19
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Hydralazine
Lymphocytes
Jurkat Cells
Antibodies
Mitogens
T-Lymphocytes
Immunologic Tests
Antihypertensive Agents
Cell Survival
B-Lymphocytes
Staining and Labeling
Antigens
Cell Line
In Vitro Techniques
Serum

Keywords

  • Antibody binding
  • Hydralazine
  • Lymphocyte

ASJC Scopus subject areas

  • Pharmacology

Cite this

Hydralazine causes nonspecific binding of antibodies to human lymphocytes in vitro. / Kucharz, Eugene J.; Goodwin, James.

In: Immunopharmacology, Vol. 19, No. 1, 1990, p. 1-4.

Research output: Contribution to journalArticle

@article{8c078f0880ab4ceb820060cbc34d39d0,
title = "Hydralazine causes nonspecific binding of antibodies to human lymphocytes in vitro",
abstract = "In vitro incubation of human lymphocytes or a continuous human Jurkat T cell line with hydralazine (1-hydrazinophthalazine), a widely used antihypertensive agent, produced a nonspecific binding of various antibodies to the cells. This phenomenon was found to produce increases in staining of mitogen-stimulated and mitogen-unstimulated human lymphocytes and Jurkat cells with a variety of antibodies recognizing T and B cell antigens. The increase in nonspecific binding was greatest at 10-2 m hydralazine, but was seen with concentrations as low 10-7 M, which is comparable to the serum concentrations of patients prescribed hydralazine. The binding of antibodies is not connected with significant decreases in the cell viability. This property of hydralazine may cause misinterpretation of certain immunological tests employing the antibodies.",
keywords = "Antibody binding, Hydralazine, Lymphocyte",
author = "Kucharz, {Eugene J.} and James Goodwin",
year = "1990",
doi = "10.1016/0162-3109(90)90020-F",
language = "English (US)",
volume = "19",
pages = "1--4",
journal = "Immunopharmacology",
issn = "0162-3109",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Hydralazine causes nonspecific binding of antibodies to human lymphocytes in vitro

AU - Kucharz, Eugene J.

AU - Goodwin, James

PY - 1990

Y1 - 1990

N2 - In vitro incubation of human lymphocytes or a continuous human Jurkat T cell line with hydralazine (1-hydrazinophthalazine), a widely used antihypertensive agent, produced a nonspecific binding of various antibodies to the cells. This phenomenon was found to produce increases in staining of mitogen-stimulated and mitogen-unstimulated human lymphocytes and Jurkat cells with a variety of antibodies recognizing T and B cell antigens. The increase in nonspecific binding was greatest at 10-2 m hydralazine, but was seen with concentrations as low 10-7 M, which is comparable to the serum concentrations of patients prescribed hydralazine. The binding of antibodies is not connected with significant decreases in the cell viability. This property of hydralazine may cause misinterpretation of certain immunological tests employing the antibodies.

AB - In vitro incubation of human lymphocytes or a continuous human Jurkat T cell line with hydralazine (1-hydrazinophthalazine), a widely used antihypertensive agent, produced a nonspecific binding of various antibodies to the cells. This phenomenon was found to produce increases in staining of mitogen-stimulated and mitogen-unstimulated human lymphocytes and Jurkat cells with a variety of antibodies recognizing T and B cell antigens. The increase in nonspecific binding was greatest at 10-2 m hydralazine, but was seen with concentrations as low 10-7 M, which is comparable to the serum concentrations of patients prescribed hydralazine. The binding of antibodies is not connected with significant decreases in the cell viability. This property of hydralazine may cause misinterpretation of certain immunological tests employing the antibodies.

KW - Antibody binding

KW - Hydralazine

KW - Lymphocyte

UR - http://www.scopus.com/inward/record.url?scp=0025189730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025189730&partnerID=8YFLogxK

U2 - 10.1016/0162-3109(90)90020-F

DO - 10.1016/0162-3109(90)90020-F

M3 - Article

VL - 19

SP - 1

EP - 4

JO - Immunopharmacology

JF - Immunopharmacology

SN - 0162-3109

IS - 1

ER -