Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

Pankaj Mogha, Ankita Srivastava, Sushant Kumar, Sreya Das, Sanjay Kureel, Alka Dwivedi, Atharva Karulkar, Nikita Jain, Abhijeet Sawant, Chitra Nayak, Abhijit Majumder, Rahul Purwar

Research output: Contribution to journalArticlepeer-review

Abstract

High numbers of autologous human primary keratinocytes (HPKs) are required for patients with burns, wounds and for gene therapy of skin disorders. Although freshly isolated HPKs exhibit a robust regenerative capacity, traditional methodology fails to provide a sufficient number of cells. Here we demonstrated a well characterized, non-cytotoxic and inert hydrogel as a substrate that mimics skin elasticity, which can accelerate proliferation and generate higher numbers of HPKs compared to existing tissue culture plastic (TCP) dishes. More importantly, this novel method was independent of feeder layer or any exogenous pharmaceutical drug. The HPKs from the hydrogel-substrate were functional as demonstrated by wound-healing assay, and the expression of IFN-γ-responsive genes (CXCL10, HLADR). Importantly, gene delivery efficiency by a lentiviral based delivery system was significantly higher in HPKs cultured on hydrogels compared with TCP. In conclusion, our study provides the first evidence that cell-material mechanical interaction is enough to provide a rapid expansion of functional keratinocytes that might be used as autologous grafts for skin disorders.

Original languageEnglish (US)
Pages (from-to)10174-10183
Number of pages10
JournalRSC Advances
Volume9
Issue number18
DOIs
StatePublished - 2019
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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