Hydrogen-exchange kinetics studied through analysis of self-decoupling of nuclear magnetic resonance

Ridvan Nepravishta, Binhan Yu, Junji Iwahara

Research output: Contribution to journalArticle

Abstract

Hydrogen exchange between solute and water molecules occurs across a wide range of timescales. Rapid hydrogen-exchange processes can effectively diminish 1H-15N scalar couplings. We demonstrate that the self-decoupling of 15N nuclear magnetic resonance can allow quantitative investigations of hydrogen exchange on a micro- to millisecond timescale, which is relatively difficult to analyze with other methods. Using a Liouvillian matrix incorporating hydrogen exchange as a mechanism for scalar relaxation, the hydrogen exchange rate can be determined from 15N NMR line shapes recorded with and without 1H decoupling. Self-decoupling offers a simple approach to analyze the kinetics of hydrogen exchange in a wide range of timescale.

Original languageEnglish (US)
Article number106687
JournalJournal of Magnetic Resonance
Volume312
DOIs
StatePublished - Mar 2020

Keywords

  • Hydrogen exchange
  • Ions
  • Kinetics
  • Nuclear magnetic resonance
  • Self-decoupling

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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