Hydrogen sulfide decreases adenosine triphosphate levels in aortic rings and leads to vasorelaxation via metabolic inhibition

Levente Kiss, Edwin A. Deitch, Csaba Szabo

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Aims: Hydrogen sulfide (H2S) at low concentrations serves as a physiological endogenous vasodilator molecule, while at higher concentrations it can trigger cytotoxic effects. The aim of our study was to elucidate the potential mechanisms responsible for the effects of H2S on vascular tone. Main methods: We measured the vascular tone in vitro in precontracted rat thoracic aortic rings and we have tested the effect of different oxygen levels and a variety of inhibitors affecting known vasodilatory pathways. We have also compared the vascular effect of high concentrations of H2S to those of pharmacological inhibitors of oxidative phosphorylation. Furthermore, we measured adenosine triphosphate (ATP)-levels in the same vascular tissues. Key findings: We have found that in rat aortic rings: (1) H2S decreases ATP levels; (2) relaxations to H2S depend on the ambient oxygen concentration; (3) prostaglandins do not take part in the H2S induced relaxations; (4) the 3':5'-cyclic guanosine monophosphate (cGMP)-nitric oxide (NO) pathway does not have a role in the relaxations (5) the role of KATP channels is limited, while Cl-/HCO3 - channels have a role in the relaxations. (6): We have observed that high concentrations of H2S relax the aortic rings in a fashion similar to sodium cyanide, and both agents reduce cellular ATP levels to a comparable degree. Significance: H2S, a new gasotransmitter of emerging importance, leads to relaxation via Cl-/HCO3 - channels and metabolic inhibition and the interactions of these two factors depend on the oxygen levels of the tissue.

Original languageEnglish (US)
Pages (from-to)589-594
Number of pages6
JournalLife Sciences
Volume83
Issue number17-18
DOIs
StatePublished - Oct 24 2008

Fingerprint

Hydrogen Sulfide
Vasodilation
Blood Vessels
Adenosine Triphosphate
Oxygen
Rats
Gasotransmitters
Sodium Cyanide
Tissue
KATP Channels
Guanosine Monophosphate
Vasodilator Agents
Prostaglandins
Oxidative Phosphorylation
Cyclic GMP
Nitric Oxide
Molecules
Thorax
Pharmacology

Keywords

  • Hydrogen sulfide
  • Oxidative phosphorylation
  • Vasorelaxation

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hydrogen sulfide decreases adenosine triphosphate levels in aortic rings and leads to vasorelaxation via metabolic inhibition. / Kiss, Levente; Deitch, Edwin A.; Szabo, Csaba.

In: Life Sciences, Vol. 83, No. 17-18, 24.10.2008, p. 589-594.

Research output: Contribution to journalArticle

Kiss, Levente ; Deitch, Edwin A. ; Szabo, Csaba. / Hydrogen sulfide decreases adenosine triphosphate levels in aortic rings and leads to vasorelaxation via metabolic inhibition. In: Life Sciences. 2008 ; Vol. 83, No. 17-18. pp. 589-594.
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