Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes

Ling Zhang, Guangdong Yang, Ashley Untereiner, Youngjun Ju, Lingyun Wu, Rui Wang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Mounting evidence has established hydrogen sulfide (H2S) as an important gasotransmitter with multifaceted physiological functions. The aim of the present study was to investigate the role of H2S on glucose utilization, glycogen synthesis, as well as gluconeogenesis in both HepG 2 cells and primary mouse hepatocytes. Incubation with NaHS (a H 2S donor) impaired glucose uptake and glycogen storage in HepG 2 cells via decreasing glucokinase activity. Adenovirus-mediated cystathionine γ-lyase (CSE) overexpression increased endogenous H 2S production and lowered glycogen content in HepG2 cells. Glycogen content was significantly higher in liver tissues from CSE knockout (KO) mice compared to that from wild type (WT) mice in fed condition. Glucose consumption was less in primarily cultured hepatocytes isolated from WT mice than those from CSE KO mice, but more glucose was produced by hepatocytes via gluconeogenesis and glycogenolysis pathways in WT mice than in CSE KO mice. NaHS treatment reduced the phosphorylation of AMP-activated protein kinase, whereas stimulation of AMP-activated protein kinase by 5-aminoimidazole-4-car-boxamide- 1-β-d-ribofuranoside reversed H2S-impaired glucose uptake. H2S-increased glucose production was likely through increased phosphoenolpyruvate carboxykinase activity. In addition, insulin at the physiological range inhibited CSE expression, and H2S decreased insulin-stimulated phosphorylation of Akt in HepG2 cells. CSE expression was increased, however, in insulin-resistant state induced by exposing cells to high levels of insulin (500 nM) and glucose (33 mM) for 24 h. Taken together, these data suggest that the interaction of H2S and insulin in liver plays a pivotal role in regulating insulin sensitivity and glucose metabolism.

Original languageEnglish (US)
Pages (from-to)114-126
Number of pages13
JournalEndocrinology
Volume154
Issue number1
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

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Hydrogen Sulfide
Gluconeogenesis
Cystathionine
Lyases
Hepatocytes
Glucose
Glycogen
Insulin
Knockout Mice
AMP-Activated Protein Kinases
Hep G2 Cells
Gasotransmitters
Phosphorylation
Glucokinase
Glycogenolysis
Phosphoenolpyruvate
Liver
Adenoviridae
Insulin Resistance

ASJC Scopus subject areas

  • Endocrinology

Cite this

Zhang, L., Yang, G., Untereiner, A., Ju, Y., Wu, L., & Wang, R. (2013). Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes. Endocrinology, 154(1), 114-126. https://doi.org/10.1210/en.2012-1658

Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes. / Zhang, Ling; Yang, Guangdong; Untereiner, Ashley; Ju, Youngjun; Wu, Lingyun; Wang, Rui.

In: Endocrinology, Vol. 154, No. 1, 01.01.2013, p. 114-126.

Research output: Contribution to journalArticle

Zhang, L, Yang, G, Untereiner, A, Ju, Y, Wu, L & Wang, R 2013, 'Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes', Endocrinology, vol. 154, no. 1, pp. 114-126. https://doi.org/10.1210/en.2012-1658
Zhang, Ling ; Yang, Guangdong ; Untereiner, Ashley ; Ju, Youngjun ; Wu, Lingyun ; Wang, Rui. / Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes. In: Endocrinology. 2013 ; Vol. 154, No. 1. pp. 114-126.
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