Hydrogen sulfide inhibits Kir2 and Kir3 channels by decreasing sensitivity to the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2)

Junghoon Ha, Yu Xu, Takeharu Kawano, Tyler Hendon, Lia Baki, Sumanta Garai, Andreas Papapetropoulos, Ganesh A. Thakur, Leigh D. Plant, Diomedes E. Logothetis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Inwardly rectifying potassium (Kir) channels establish and regulate the resting membrane potential of excitable cells in the heart, brain, and other peripheral tissues. Phosphatidylinositol 4,5-bisphosphate (PIP2) is a key direct activator of ion channels, including Kir channels. The gasotransmitter carbon monoxide has been shown to regulate Kir channel activity by altering channel–PIP2 interactions. Here, we tested in two cellular models the effects and mechanism of action of another gasotransmitter, hydrogen sulfide (H2S), thought to play a key role in cellular responses under ischemic conditions. Direct administration of sodium hydrogen sulfide as an exogenous H2S source and expression of cystathionine -lyase, a key enzyme that produces endogenous H2S in specific brain tissues, resulted in comparable current inhibition of several Kir2 and Kir3 channels. This effect resulted from changes in channel-gating kinetics rather than in conductance or cell-surface localization. The extent of H2S regulation depended on the strength of the channel–PIP2 interactions. H2S regulation was attenuated when channel–PIP2 interactions were strengthened and was increased when channel–PIP2 interactions were weakened by depleting PIP2 levels. These H2S effects required specific cytoplasmic cysteine residues in Kir3.2 channels. Mutation of these residues abolished H2S inhibition, and reintroduction of specific cysteine residues back into the background of the cytoplasmic cysteine-lacking mutant rescued H2S inhibition. Molecular dynamics simulation experiments provided mechanistic insights into how potential sulfhydration of specific cysteine residues could lead to changes in channel–PIP2 interactions and channel gating.

Original languageEnglish (US)
Pages (from-to)3546-3561
Number of pages16
JournalJournal of Biological Chemistry
Volume293
Issue number10
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

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Hydrogen Sulfide
Phosphatidylinositols
Cysteine
Phospholipids
Gasotransmitters
Brain
Cystathionine
Tissue
Inwardly Rectifying Potassium Channel
Lyases
Molecular Dynamics Simulation
Carbon Monoxide
Ion Channels
Membrane Potentials
Molecular dynamics
Potassium
Sodium
Membranes
Mutation
Kinetics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hydrogen sulfide inhibits Kir2 and Kir3 channels by decreasing sensitivity to the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2). / Ha, Junghoon; Xu, Yu; Kawano, Takeharu; Hendon, Tyler; Baki, Lia; Garai, Sumanta; Papapetropoulos, Andreas; Thakur, Ganesh A.; Plant, Leigh D.; Logothetis, Diomedes E.

In: Journal of Biological Chemistry, Vol. 293, No. 10, 01.01.2018, p. 3546-3561.

Research output: Contribution to journalArticle

Ha, Junghoon ; Xu, Yu ; Kawano, Takeharu ; Hendon, Tyler ; Baki, Lia ; Garai, Sumanta ; Papapetropoulos, Andreas ; Thakur, Ganesh A. ; Plant, Leigh D. ; Logothetis, Diomedes E. / Hydrogen sulfide inhibits Kir2 and Kir3 channels by decreasing sensitivity to the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2). In: Journal of Biological Chemistry. 2018 ; Vol. 293, No. 10. pp. 3546-3561.
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AU - Kawano, Takeharu

AU - Hendon, Tyler

AU - Baki, Lia

AU - Garai, Sumanta

AU - Papapetropoulos, Andreas

AU - Thakur, Ganesh A.

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