Hydrogen sulfide replacement therapy protects the vascular endothelium in hyperglycemia by preserving mitochondrial function

Kunihiro Suzuki, Gabor Olah, Katalin Modis, Ciro Coletta, Gabriella Kulp, Domokos Gerö, Petra Szoleczky, Tuanjie Chang, Zongmin Zhou, Lingyun Wu, Rui Wang, Andreas Papapetropoulos, Csaba Szabo

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

The goal of the present studies was to investigate the role of Changes in hydrogen sulfide (H 2S) homeostasis in the pathogenesis of hyperglycemic endothelial dysfunction. Exposure of bEnd3 microvascular endothelial cells to elevated extracellular glucose (in vitro "hyperglycemia") induced the mitochondrial formation of reactive oxygen species (ROS), which resulted in an increased consumption of endogenous and exogenous H 2S. Replacement of H 2S or overexpression of the H 2S-producing enzyme cystathionine-γ-lyase (CSE) attenuated the hyperglycemia-induced enhancement of ROS formation, attenuated nuclear DNA injury, reduced the activation of the nuclear enzyme poly(ADP-ribose) polymerase, and improved cellular viability. In vitro hyperglycemia resulted in a switch from oxidative phosphorylation to glycolysis, an effect that was partially corrected by H 2S supplementation. Exposure of isolated vascular rings to high glucose in vitro induced an impairment of endothelium-dependent relaxations, which was prevented by CSE overexpression or H 2S supplementation. siRNA silencing of CSE exacerbated ROS production in hyperglycemic endothelial cells. Vascular rings from CSE -/- mice exhibited an accelerated impairment of endothelium-dependent relaxations in response to in vitro hyperglycemia, compared with wild-type controls. Streptozotocin-induced diabetes in rats resulted in a decrease in the circulating level of H 2S; replacement of H 2S protected from the development of endothelial dysfunction ex vivo. In conclusion, endogenously produced H 2S protects against the development of hyperglycemia-induced endothelial dysfunction. We hypothesize that, in hyperglycemic endothelial cells, mitochondrial ROS production and increased H 2S catabolismform a positive feed-forward cycle. H 2S replacement protects against these alterations, resulting in reduced ROS formation, improved endothelial metabolic state, and maintenance of normal endothelial function.

Original languageEnglish (US)
Pages (from-to)13829-13834
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number33
DOIs
StatePublished - Aug 16 2011

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Hydrogen Sulfide
Cystathionine
Vascular Endothelium
Lyases
Hyperglycemia
Reactive Oxygen Species
Endothelial Cells
Endothelium
Blood Vessels
Therapeutics
Glucose
Enzyme Activation
Experimental Diabetes Mellitus
Poly(ADP-ribose) Polymerases
Oxidative Phosphorylation
Glycolysis
Small Interfering RNA
DNA Damage
Homeostasis
Maintenance

ASJC Scopus subject areas

  • General

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Hydrogen sulfide replacement therapy protects the vascular endothelium in hyperglycemia by preserving mitochondrial function. / Suzuki, Kunihiro; Olah, Gabor; Modis, Katalin; Coletta, Ciro; Kulp, Gabriella; Gerö, Domokos; Szoleczky, Petra; Chang, Tuanjie; Zhou, Zongmin; Wu, Lingyun; Wang, Rui; Papapetropoulos, Andreas; Szabo, Csaba.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 33, 16.08.2011, p. 13829-13834.

Research output: Contribution to journalArticle

Suzuki, Kunihiro ; Olah, Gabor ; Modis, Katalin ; Coletta, Ciro ; Kulp, Gabriella ; Gerö, Domokos ; Szoleczky, Petra ; Chang, Tuanjie ; Zhou, Zongmin ; Wu, Lingyun ; Wang, Rui ; Papapetropoulos, Andreas ; Szabo, Csaba. / Hydrogen sulfide replacement therapy protects the vascular endothelium in hyperglycemia by preserving mitochondrial function. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 33. pp. 13829-13834.
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AU - Gerö, Domokos

AU - Szoleczky, Petra

AU - Chang, Tuanjie

AU - Zhou, Zongmin

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AU - Papapetropoulos, Andreas

AU - Szabo, Csaba

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