Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion

Margaret Das, Audrey Hart-Van Tassell, Petri T. Urvil, Susan Lea, David Pettigrew, K. L. Anderson, Alfred Samet, Jozef Kur, Steve Matthews, Stella Nowicki, Vsevolod Popov, Pawel Goluszko, Bogdan J. Nowicki

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Uropathogenic and diarrheal Escherichia coli strains expressing adhesins of the Dr family bind to decay-accelerating factor, invade epithelial cells, preferentially infect children and pregnant women, and may be associated with chronic or recurrent infections. Thus far, no fimbrial domain(s) that facilitates cell invasion has been identified. We used alanine scanning mutagenesis to replace selected amino acids in hydrophilic domain II of the structural fimbrial subunit DraE and evaluated recombinant mutant DraE for attachment, invasion, and intracellular compartmentalization. The mutation of amino acids V28, T31, G33, Q34, T36, and P40 of DraE reduced or abolished HeLa cell invasion but did not affect attachment. Electron micrographs showed a stepwise entry and fusion of vacuoles containing Escherichia coli mutants T36A and Q34A or corresponding beads with lysosomes, whereas vacuoles with wild-type Dr adhesin showed no fusion. Mutants T31A and Q34A, which were deficient in invasion, appeared to display a reduced capacity for clustering decay-accelerating factor. Our findings suggest that hydrophilic domain II may be involved in cell entry. These data are consistent with the interpretation that in HeLa cells the binding and invasion phenotypes of Dr fimbriae may be separated.

Original languageEnglish (US)
Pages (from-to)6119-6126
Number of pages8
JournalInfection and Immunity
Volume73
Issue number9
DOIs
StatePublished - Sep 2005

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CD55 Antigens
Vacuoles
HeLa Cells
Uropathogenic Escherichia coli
Escherichia coli
Amino Acids
Lysosomes
Mutagenesis
Alanine
Cluster Analysis
Pregnant Women
Epithelial Cells
Electrons
Phenotype
Mutation
Infection
adjuvant P40

ASJC Scopus subject areas

  • Immunology

Cite this

Das, M., Hart-Van Tassell, A., Urvil, P. T., Lea, S., Pettigrew, D., Anderson, K. L., ... Nowicki, B. J. (2005). Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion. Infection and Immunity, 73(9), 6119-6126. https://doi.org/10.1128/IAI.73.9.6119-6126.2005

Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion. / Das, Margaret; Hart-Van Tassell, Audrey; Urvil, Petri T.; Lea, Susan; Pettigrew, David; Anderson, K. L.; Samet, Alfred; Kur, Jozef; Matthews, Steve; Nowicki, Stella; Popov, Vsevolod; Goluszko, Pawel; Nowicki, Bogdan J.

In: Infection and Immunity, Vol. 73, No. 9, 09.2005, p. 6119-6126.

Research output: Contribution to journalArticle

Das, M, Hart-Van Tassell, A, Urvil, PT, Lea, S, Pettigrew, D, Anderson, KL, Samet, A, Kur, J, Matthews, S, Nowicki, S, Popov, V, Goluszko, P & Nowicki, BJ 2005, 'Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion', Infection and Immunity, vol. 73, no. 9, pp. 6119-6126. https://doi.org/10.1128/IAI.73.9.6119-6126.2005
Das M, Hart-Van Tassell A, Urvil PT, Lea S, Pettigrew D, Anderson KL et al. Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion. Infection and Immunity. 2005 Sep;73(9):6119-6126. https://doi.org/10.1128/IAI.73.9.6119-6126.2005
Das, Margaret ; Hart-Van Tassell, Audrey ; Urvil, Petri T. ; Lea, Susan ; Pettigrew, David ; Anderson, K. L. ; Samet, Alfred ; Kur, Jozef ; Matthews, Steve ; Nowicki, Stella ; Popov, Vsevolod ; Goluszko, Pawel ; Nowicki, Bogdan J. / Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion. In: Infection and Immunity. 2005 ; Vol. 73, No. 9. pp. 6119-6126.
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