Hyper-recombination and mutator effects of the mms9-1, mms13-1, and mms21-1 mutations in Saccharomyces cerevisiae

Beth A. Montelone, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Spontaneous mitotic intragenic and intergenic recombination at various sites is enhanced 10 to 100 fold in the methyl methanesulfonate (MMS)-sensitive mutants mms9-1, mms13-1, and mms21-1 of Saccharomyces cerevisiae. All three mutants show elevated rates of spontaneous mutation. Sporulation is reduced in diploids homozygous for any of the three mutations, and a deficiency in meiotic recombination and meiotic chromosome segregation is observed. Pleiotropic effects on cell viability, growth rate, and radiation sensitivity, in combination with the alterations in recombination and mutagenesis displayed by mutant strains, suggest that the MMS9, MMS13, and MMS21 genes play important roles in DNA replication and/or genetic recombination.

Original languageEnglish (US)
Pages (from-to)223-232
Number of pages10
JournalCurrent Genetics
Volume4
Issue number3
DOIs
StatePublished - Dec 1 1981
Externally publishedYes

Keywords

  • Hyper-recombination mutants
  • Meiotic recombination
  • Mitotic recombination
  • Mutators

ASJC Scopus subject areas

  • Genetics

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