Hypercortisolemia alters muscle protein anabolism following ingestion of essential amino acids

Douglas Paddon-Jones, Melinda Sheffield-Moore, Daniel L. Creson, Arthur P. Sanford, Steven Wolf, Robert R. Wolfe, Arny A. Ferrando

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Debilitating injury is accompanied by hypercortisolemia, muscle wasting, and disruption of the normal anabolic response to food. We sought to determine whether acute hypercortisolemia alters muscle protein metabolism following ingestion of a potent anabolic stimulus: essential amino acids (EAA). A 27-h infusion (80 μg.kg-1.h-1) of hydrocortisone sodium succinate mimicked cortisol (C) levels accompanying severe injury (>30 μg/dl), (C + AA; n = 6). The control group (AA) received intravenous saline (n = 6). Femoral arteriovenous blood samples and muscle biopsies were obtained during a primed (2.0 μmol/kg) constant infusion (0.05 μmol·kg-1·min-1 of L-[ring-2H5]phenylalanine before and after ingestion of 15 g of EAA. Hypercortisolemia [36.5 ± 2.1 (C + AA) vs. 9.0 ± 1.0 μg/dl (AA)] increased postabsorptive arterial, venous, and muscle intracellular phenylalanine concentrations. Hypercortisolemia also increased postabsorptive and post-EAA insulin concentrations. Net protein balance was blunted (40% lower) following EAA ingestion but remained positive for a greater period of time (60 vs. 180 min) in the C + AA group. Thus, although differences in protein metabolism were evident, EAA ingestion improved muscle protein anabolism during acute hypercortisolemia and may help minimize muscle loss following debilitating injury.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume284
Issue number5 47-5
StatePublished - May 1 2003

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Essential Amino Acids
Muscle Proteins
Eating
Muscle
Muscles
Phenylalanine
Metabolism
Hydrocortisone
Wounds and Injuries
Biopsy
Succinic Acid
Thigh
Proteins
Blood
Sodium
Insulin
Food
Control Groups

Keywords

  • Cortisol
  • Injury
  • Metabolism
  • Stable isotopes
  • Stress

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Hypercortisolemia alters muscle protein anabolism following ingestion of essential amino acids. / Paddon-Jones, Douglas; Sheffield-Moore, Melinda; Creson, Daniel L.; Sanford, Arthur P.; Wolf, Steven; Wolfe, Robert R.; Ferrando, Arny A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 284, No. 5 47-5, 01.05.2003.

Research output: Contribution to journalArticle

Paddon-Jones, Douglas ; Sheffield-Moore, Melinda ; Creson, Daniel L. ; Sanford, Arthur P. ; Wolf, Steven ; Wolfe, Robert R. ; Ferrando, Arny A. / Hypercortisolemia alters muscle protein anabolism following ingestion of essential amino acids. In: American Journal of Physiology - Endocrinology and Metabolism. 2003 ; Vol. 284, No. 5 47-5.
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