Hyperglycemia-induced inhibition of splanchnic fatty acid oxidation increases hepatic triacylglycerol secretion

Labros S. Sidossis, Bettina Mittendorfer, Eric Walser, David Chinkes, Robert R. Wolfe

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The effect of hyperglycemia (~8 mmol/l) on splanchnic fatty acid oxidation and triacylglycerol (TG) secretion rates was investigated in five healthy men. U-13C-labeled fatty acids were infused to estimate fatty acid kinetics and oxidation across the splanchnic region, and in vivo labeled very low density lipoprotein (VLDL)-TG was infused to estimate TG secretion rate. Plasma fatty acid carbon enrichment and concentration were maintained constant by infusion of lipids and heparin in the hyperglycemia experiments. Fatty acid uptake by the splanchnic region was 1.4 ± 0.2 and 2.2 ± 0.9 μmol · kg-1 · min-1 in the basal and clamp experiments, respectively, whereas fatty acid oxidation decreased from 0.4 ± 0.04 to 0.2 ± 0.05 μmol · kg-1 · min-1 (P < 0.05). Hepatic TG secretion increased from 0.35 ± 0.07 μmol · kg-1 · min-1 in the basal state to 0.53 ± 0.11 μmol · kg-1 · min-1 after 15 h of hyperglycemia (P < 0.05). Similarly, plasma VLDL-TG concentration increased from 0.28 ± 0.06 to 0.43 ± 0.05 mmol/l during the clamp (P < 0.05). In summary, hyperglycemia attenuates fatty acid oxidation in the splanchnic region in human volunteers, even when fatty acid availability is constant. This adaptation results in a significant increase in the VLDL-TG secretion rate and concentration in plasma.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume275
Issue number5 38-5
StatePublished - 1998
Externally publishedYes

Fingerprint

Viscera
Hyperglycemia
Triglycerides
Fatty Acids
Oxidation
Liver
VLDL Lipoproteins
Clamping devices
Plasmas
Heparin
Volunteers
Carbon
Experiments
Availability
Lipids
Kinetics

Keywords

  • Blood flow
  • Carnitine palmitoyltransferase
  • Diabetes
  • Hepatic vein
  • Liver
  • Malonyl-coenzyme A
  • Obesity

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Hyperglycemia-induced inhibition of splanchnic fatty acid oxidation increases hepatic triacylglycerol secretion. / Sidossis, Labros S.; Mittendorfer, Bettina; Walser, Eric; Chinkes, David; Wolfe, Robert R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 275, No. 5 38-5, 1998.

Research output: Contribution to journalArticle

@article{72b5327f5c5d4c08ad201439ad581b1a,
title = "Hyperglycemia-induced inhibition of splanchnic fatty acid oxidation increases hepatic triacylglycerol secretion",
abstract = "The effect of hyperglycemia (~8 mmol/l) on splanchnic fatty acid oxidation and triacylglycerol (TG) secretion rates was investigated in five healthy men. U-13C-labeled fatty acids were infused to estimate fatty acid kinetics and oxidation across the splanchnic region, and in vivo labeled very low density lipoprotein (VLDL)-TG was infused to estimate TG secretion rate. Plasma fatty acid carbon enrichment and concentration were maintained constant by infusion of lipids and heparin in the hyperglycemia experiments. Fatty acid uptake by the splanchnic region was 1.4 ± 0.2 and 2.2 ± 0.9 μmol · kg-1 · min-1 in the basal and clamp experiments, respectively, whereas fatty acid oxidation decreased from 0.4 ± 0.04 to 0.2 ± 0.05 μmol · kg-1 · min-1 (P < 0.05). Hepatic TG secretion increased from 0.35 ± 0.07 μmol · kg-1 · min-1 in the basal state to 0.53 ± 0.11 μmol · kg-1 · min-1 after 15 h of hyperglycemia (P < 0.05). Similarly, plasma VLDL-TG concentration increased from 0.28 ± 0.06 to 0.43 ± 0.05 mmol/l during the clamp (P < 0.05). In summary, hyperglycemia attenuates fatty acid oxidation in the splanchnic region in human volunteers, even when fatty acid availability is constant. This adaptation results in a significant increase in the VLDL-TG secretion rate and concentration in plasma.",
keywords = "Blood flow, Carnitine palmitoyltransferase, Diabetes, Hepatic vein, Liver, Malonyl-coenzyme A, Obesity",
author = "Sidossis, {Labros S.} and Bettina Mittendorfer and Eric Walser and David Chinkes and Wolfe, {Robert R.}",
year = "1998",
language = "English (US)",
volume = "275",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "5 38-5",

}

TY - JOUR

T1 - Hyperglycemia-induced inhibition of splanchnic fatty acid oxidation increases hepatic triacylglycerol secretion

AU - Sidossis, Labros S.

AU - Mittendorfer, Bettina

AU - Walser, Eric

AU - Chinkes, David

AU - Wolfe, Robert R.

PY - 1998

Y1 - 1998

N2 - The effect of hyperglycemia (~8 mmol/l) on splanchnic fatty acid oxidation and triacylglycerol (TG) secretion rates was investigated in five healthy men. U-13C-labeled fatty acids were infused to estimate fatty acid kinetics and oxidation across the splanchnic region, and in vivo labeled very low density lipoprotein (VLDL)-TG was infused to estimate TG secretion rate. Plasma fatty acid carbon enrichment and concentration were maintained constant by infusion of lipids and heparin in the hyperglycemia experiments. Fatty acid uptake by the splanchnic region was 1.4 ± 0.2 and 2.2 ± 0.9 μmol · kg-1 · min-1 in the basal and clamp experiments, respectively, whereas fatty acid oxidation decreased from 0.4 ± 0.04 to 0.2 ± 0.05 μmol · kg-1 · min-1 (P < 0.05). Hepatic TG secretion increased from 0.35 ± 0.07 μmol · kg-1 · min-1 in the basal state to 0.53 ± 0.11 μmol · kg-1 · min-1 after 15 h of hyperglycemia (P < 0.05). Similarly, plasma VLDL-TG concentration increased from 0.28 ± 0.06 to 0.43 ± 0.05 mmol/l during the clamp (P < 0.05). In summary, hyperglycemia attenuates fatty acid oxidation in the splanchnic region in human volunteers, even when fatty acid availability is constant. This adaptation results in a significant increase in the VLDL-TG secretion rate and concentration in plasma.

AB - The effect of hyperglycemia (~8 mmol/l) on splanchnic fatty acid oxidation and triacylglycerol (TG) secretion rates was investigated in five healthy men. U-13C-labeled fatty acids were infused to estimate fatty acid kinetics and oxidation across the splanchnic region, and in vivo labeled very low density lipoprotein (VLDL)-TG was infused to estimate TG secretion rate. Plasma fatty acid carbon enrichment and concentration were maintained constant by infusion of lipids and heparin in the hyperglycemia experiments. Fatty acid uptake by the splanchnic region was 1.4 ± 0.2 and 2.2 ± 0.9 μmol · kg-1 · min-1 in the basal and clamp experiments, respectively, whereas fatty acid oxidation decreased from 0.4 ± 0.04 to 0.2 ± 0.05 μmol · kg-1 · min-1 (P < 0.05). Hepatic TG secretion increased from 0.35 ± 0.07 μmol · kg-1 · min-1 in the basal state to 0.53 ± 0.11 μmol · kg-1 · min-1 after 15 h of hyperglycemia (P < 0.05). Similarly, plasma VLDL-TG concentration increased from 0.28 ± 0.06 to 0.43 ± 0.05 mmol/l during the clamp (P < 0.05). In summary, hyperglycemia attenuates fatty acid oxidation in the splanchnic region in human volunteers, even when fatty acid availability is constant. This adaptation results in a significant increase in the VLDL-TG secretion rate and concentration in plasma.

KW - Blood flow

KW - Carnitine palmitoyltransferase

KW - Diabetes

KW - Hepatic vein

KW - Liver

KW - Malonyl-coenzyme A

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=0031731373&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031731373&partnerID=8YFLogxK

M3 - Article

VL - 275

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 5 38-5

ER -