TY - JOUR
T1 - Hyperoxia by short-term promotes oxidative damage and mitochondrial dysfunction in rat brain
AU - Machado, Richard Simon
AU - Tenfen, Leonardo
AU - Joaquim, Larissa
AU - Lanzzarin, Everton Venicius Rosa
AU - Bernardes, Gabriela Costa
AU - Bonfante, Sandra Regina
AU - Mathias, Khiany
AU - Biehl, Erica
AU - Bagio, Érick
AU - Stork, Solange de Souza
AU - Denicol, Tais
AU - de Oliveira, Mariana Pacheco
AU - da Silva, Mariella Reinol
AU - Danielski, Lucinéia Gainski
AU - de Quadros, Rafaella Willig
AU - Rezin, Gislaine Tezza
AU - Terra, Silvia Resende
AU - Balsini, Jairo Nunes
AU - Gava, Fernanda Frederico
AU - Petronilho, Fabricia
N1 - Publisher Copyright:
© 2022
PY - 2022/12
Y1 - 2022/12
N2 - Oxygen (O2) therapy is used as a therapeutic protocol to prevent or treat hypoxia. However, a high inspired fraction of O2 (FIO2) promotes hyperoxia, a harmful condition for the central nervous system (CNS). The present study evaluated parameters of oxidative stress and mitochondrial dysfunction in the brain of rats exposed to different FIO2. Male Wistar rats were exposed to hyperoxia (FIO2 40 % and 60 %) compared to the control group (FIO2 21 %) for 2 h. Oxidative stress, neutrophilic infiltration, and mitochondrial respiratory chain enzymes were determined in the hippocampus, striatum, cerebellum, cortex, and prefrontal cortex after O2 exposure. The animals exposed to hyperoxia showed increased lipid peroxidation, formation of carbonyl proteins, N/N concentration, and neutrophilic infiltration in some brain regions, like hippocampus, striatum, and cerebellum being the most affected. Furthermore, CAT activity and activity of mitochondrial enzyme complexes were also altered after exposure to hyperoxia. Rats exposed to hyperoxia showed increase in oxidative stress parameters and mitochondrial dysfunction in brain structures.
AB - Oxygen (O2) therapy is used as a therapeutic protocol to prevent or treat hypoxia. However, a high inspired fraction of O2 (FIO2) promotes hyperoxia, a harmful condition for the central nervous system (CNS). The present study evaluated parameters of oxidative stress and mitochondrial dysfunction in the brain of rats exposed to different FIO2. Male Wistar rats were exposed to hyperoxia (FIO2 40 % and 60 %) compared to the control group (FIO2 21 %) for 2 h. Oxidative stress, neutrophilic infiltration, and mitochondrial respiratory chain enzymes were determined in the hippocampus, striatum, cerebellum, cortex, and prefrontal cortex after O2 exposure. The animals exposed to hyperoxia showed increased lipid peroxidation, formation of carbonyl proteins, N/N concentration, and neutrophilic infiltration in some brain regions, like hippocampus, striatum, and cerebellum being the most affected. Furthermore, CAT activity and activity of mitochondrial enzyme complexes were also altered after exposure to hyperoxia. Rats exposed to hyperoxia showed increase in oxidative stress parameters and mitochondrial dysfunction in brain structures.
KW - Brain
KW - Hyperoxia
KW - Oxidative stress
KW - Oxygen
UR - https://www.scopus.com/pages/publications/85138556176
UR - https://www.scopus.com/pages/publications/85138556176#tab=citedBy
U2 - 10.1016/j.resp.2022.103963
DO - 10.1016/j.resp.2022.103963
M3 - Article
C2 - 36041716
AN - SCOPUS:85138556176
SN - 1569-9048
VL - 306
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
M1 - 103963
ER -