Hyperoxia increases AP-1 DNA binding in rat brain

LiQi Tong, Tracy Toliver-Kinsky, David Rassin, Karin Werrbach-Perez, J. Regino Perez-Polo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Oxidative stress appears to contribute to neurodegenerative outcomes after ischemia, hypoxia, and hyperoxia. The AP-1 transcription factor is made up of a family of regulatory proteins that can be activated by oxidative stress. In the present study, we examined AP-1 DNA binding activity in terms of specific participating AP-1 proteins in rat brain after hyperoxia. Male Sprague-Dawley rats were exposed to 100% oxygen under isobaric conditions over time. The AP-1 DNA binding activity present in the rat hippocampus and basal forebrain was characterized by electrophoretic mobility shift analysis (EMSA) and the participating AP-1 proteins identified by immunodepletion/supershift and Western blotting analyses. The Fos and Jun proteins were localized by immunohistochemistry to hippocampus. There were significant increases in AP-1 DNA binding in both hippocampus and basal forebrain after hyperoxia. There was also a significant increase in c-Jun protein levels and the proportion of c-Jun present in AP-1 DNA binding complexes in hippocampal nuclei after hyperoxia. These results suggest that AP-1 activation via c-Jun binding to DNA is an important component of brain responses to oxidative stress.

Original languageEnglish (US)
Pages (from-to)111-115
Number of pages5
JournalNeurochemical Research
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

Hyperoxia
Transcription Factor AP-1
Rats
Brain
DNA
Oxidative stress
Hippocampus
Oxidative Stress
Proteins
Proto-Oncogene Proteins c-jun
Electrophoretic mobility
Sprague Dawley Rats
Ischemia
Western Blotting
Chemical activation
Immunohistochemistry
Oxygen

Keywords

  • AP-I
  • Basal forebrain
  • c-Jun
  • Hippocampus
  • Hyperoxia
  • Oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Tong, L., Toliver-Kinsky, T., Rassin, D., Werrbach-Perez, K., & Perez-Polo, J. R. (2003). Hyperoxia increases AP-1 DNA binding in rat brain. Neurochemical Research, 28(1), 111-115. https://doi.org/10.1023/A:1021656430576

Hyperoxia increases AP-1 DNA binding in rat brain. / Tong, LiQi; Toliver-Kinsky, Tracy; Rassin, David; Werrbach-Perez, Karin; Perez-Polo, J. Regino.

In: Neurochemical Research, Vol. 28, No. 1, 01.01.2003, p. 111-115.

Research output: Contribution to journalArticle

Tong, L, Toliver-Kinsky, T, Rassin, D, Werrbach-Perez, K & Perez-Polo, JR 2003, 'Hyperoxia increases AP-1 DNA binding in rat brain', Neurochemical Research, vol. 28, no. 1, pp. 111-115. https://doi.org/10.1023/A:1021656430576
Tong, LiQi ; Toliver-Kinsky, Tracy ; Rassin, David ; Werrbach-Perez, Karin ; Perez-Polo, J. Regino. / Hyperoxia increases AP-1 DNA binding in rat brain. In: Neurochemical Research. 2003 ; Vol. 28, No. 1. pp. 111-115.
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