Hypomethylation of DNA in estrogen-induced and -dependent hamster kidney tumors

Leejane Lu, Joachim G. Liehr, David A. Sirbasku, Erika Randerath, Kurt Randerath

Research output: Contribution to journalArticle

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Abstract

The development and maintenance of DNA hypomethylation were investigated in male Syrian hamsters during the course of induction of renal carcinoma by estrogens and in an estrogen-dependent tumor derived from H-301 cells. The H-301 cell line was obtained from a primary renal carcinoma induced by E-diethylstilbestrol treatment. Covalent DNA modifications in estrogen-exposed kidney and tumor tissues were also examined. The five tumors investigated were induced by s.c. estrogen treatment of animals for 7-9 months. Covalent DNA adducts were detected in kidneys after 5-7 months of exposure to various estrogens, but not in primary tumors induced by estrogen treatment for 7-9 months. Estrogen-induced covalent DNA modifications likewise were not detectable in tumors grown in estrogenized hamsters inoculated with H-301 cells. In contrast, DNA was hypomethylated in primary tumors induced by E-diethylstilbestrol, estradiol or 11β-ethyl-l7α-ethinyl estradiol, but not in untreated and estrogen-exposed kidney. Compared with kidney tissue, there was an 11-24% decrease in total genomic DNA methylation in the estrogen-induced and -dependent tumors. DNA hypomethylation was maintained in tumors derived from H-301 cells. Discontinuation of estrogen treatment rapidly decreased the size of estrogen-dependent H-301 tumors, but did not affect the degree of DNA hypomethylation. Thus, DNA hypomethylation occurred in hormone-dependent primary neoplasms and was maintained after serial transplantations independent of the growth status.

Original languageEnglish (US)
Pages (from-to)925-929
Number of pages5
JournalCarcinogenesis
Volume9
Issue number6
DOIs
StatePublished - Jun 1988
Externally publishedYes

Fingerprint

Estrogen
Kidney
Cricetinae
Tumors
Tumor
Estrogens
DNA
Dependent
Neoplasms
Estradiol
Diethylstilbestrol
Cell
Hormone-Dependent Neoplasms
Hamster
Tissue
Carcinoma
Ethinyl Estradiol
Transplantation
DNA Adducts
Mesocricetus

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Hypomethylation of DNA in estrogen-induced and -dependent hamster kidney tumors. / Lu, Leejane; Liehr, Joachim G.; Sirbasku, David A.; Randerath, Erika; Randerath, Kurt.

In: Carcinogenesis, Vol. 9, No. 6, 06.1988, p. 925-929.

Research output: Contribution to journalArticle

Lu, L, Liehr, JG, Sirbasku, DA, Randerath, E & Randerath, K 1988, 'Hypomethylation of DNA in estrogen-induced and -dependent hamster kidney tumors', Carcinogenesis, vol. 9, no. 6, pp. 925-929. https://doi.org/10.1093/carcin/9.6.925
Lu, Leejane ; Liehr, Joachim G. ; Sirbasku, David A. ; Randerath, Erika ; Randerath, Kurt. / Hypomethylation of DNA in estrogen-induced and -dependent hamster kidney tumors. In: Carcinogenesis. 1988 ; Vol. 9, No. 6. pp. 925-929.
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