Hypoproteinemia does not alter plasma volume expansion in response to a 0.9% saline bolus in awake sheep

Kirk I. Brauer, Lance P. Brauer, Donald S. Prough, Peter Rodhe, Robert G. Hahn, Daniel L. Traber, Lilian D. Traber, Christer H. Svensen

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Objective: To test the hypothesis that hypoproteinemia reduces plasma volume expansion produced by a bolus of crystalloid solution given to awake sheep. Design: Prospective and observational. Setting: Laboratory. Subjects: Five female merino sheep (n = 5) weighing 37 ± 3 kg were anesthetized. Interventions: Each animal was subjected to a 5-day test period: day 1: 50 mL/min 0.9% saline infusion over 20 mins. Days 2-4: daily plasmapheresis and replacement of the shed plasma with 6 L of 0.9% saline were performed in increments. Measurements and main results: Fractional plasma volume expansion after rapid infusion of saline on days 1 and 5 was calculated from changes in hemoglobin concentration. There was a significant reduction in total plasma protein concentration after plasmapheresis (p <.05). Colloid osmotic pressures were also significantly lowered (p <.05). A crystalloid infusion of 0.9% saline did not alter any of these values compared with baseline. The hemodynamic measurements did not show significant differences between the experiments. The plasma volume expansion reached approximately 20% at the end of infusion and stayed at 10-15% during the experiments. No difference was found in plasma volume expansion produced by a bolus of 50 mL/min of 0.9% in the hypoproteinemic state when compared with the euproteinemic state (p =.61). No difference in cumulative urinary output was found between the two states. Conclusions: In contrast to our hypothesis, severe acute hypoproteinemia does not reduce plasma volume expansion in response to 50 mL/min 0.9% saline infusion in nonspleenectomized sheep when compared with the resultant plasma volume expansion after a 50 mL/min of 0.9% infusion in the euproteinemic state.

Original languageEnglish (US)
Pages (from-to)2011-2015
Number of pages5
JournalCritical care medicine
Issue number10
StatePublished - Oct 2010
Externally publishedYes


  • anesthesia
  • crystalloids
  • fluid distribution
  • hypoproteinemia
  • plasma volume expansion

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine


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