TY - JOUR
T1 - i-BLESS is an ultra-sensitive method for detection of DNA double-strand breaks
AU - Biernacka, Anna
AU - Zhu, Yingjie
AU - Skrzypczak, Magdalena
AU - Forey, Romain
AU - Pardo, Benjamin
AU - Grzelak, Marta
AU - Nde, Jules
AU - Mitra, Abhishek
AU - Kudlicki, Andrzej
AU - Crosetto, Nicola
AU - Pasero, Philippe
AU - Rowicka, Maga
AU - Ginalski, Krzysztof
N1 - Funding Information:
This work was supported by Polish National Science Centre (2011/02/A/NZ2/00014 to K. G. and 2015/17/D/NZ2/03711 to M.S.) and Foundation for Polish Science (TEAM to K. G.). Funding to M.R., Y.Z., A.M., J.N. and A.K. was provided by the NIH grant (5 R01 GM 112131 to M.R.). Funding to P.P. was provided by ANR, INCa, MSD Avenir Fund and Ligue contre le Cancer. N.C. is supported by the Karolinska Institutet, the Swedish Research Council, the Swedish Cancer Research Foundation, the Ragnar Söderberg Foundation, and the Swedish Foundation for Strategic Research.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Maintenance of genome stability is a key issue for cell fate that could be compromised by chromosome deletions and translocations caused by DNA double-strand breaks (DSBs). Thus development of precise and sensitive tools for DSBs labeling is of great importance for understanding mechanisms of DSB formation, their sensing and repair. Until now there has been no high resolution and specific DSB detection technique that would be applicable to any cells regardless of their size. Here, we present i-BLESS, a universal method for direct genome-wide DNA double-strand break labeling in cells immobilized in agarose beads. i-BLESS has three key advantages: it is the only unbiased method applicable to yeast, achieves a sensitivity of one break at a given position in 100,000 cells, and eliminates background noise while still allowing for fixation of samples. The method allows detection of ultra-rare breaks such as those forming spontaneously at G-quadruplexes.
AB - Maintenance of genome stability is a key issue for cell fate that could be compromised by chromosome deletions and translocations caused by DNA double-strand breaks (DSBs). Thus development of precise and sensitive tools for DSBs labeling is of great importance for understanding mechanisms of DSB formation, their sensing and repair. Until now there has been no high resolution and specific DSB detection technique that would be applicable to any cells regardless of their size. Here, we present i-BLESS, a universal method for direct genome-wide DNA double-strand break labeling in cells immobilized in agarose beads. i-BLESS has three key advantages: it is the only unbiased method applicable to yeast, achieves a sensitivity of one break at a given position in 100,000 cells, and eliminates background noise while still allowing for fixation of samples. The method allows detection of ultra-rare breaks such as those forming spontaneously at G-quadruplexes.
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U2 - 10.1038/s42003-018-0165-9
DO - 10.1038/s42003-018-0165-9
M3 - Article
C2 - 30393778
AN - SCOPUS:85060685266
SN - 2399-3642
VL - 1
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 181
ER -