Ia specific antilymphocyte antibodies in rheumatoid arthritis

R. P. Searles, K. Okudaira, S. M. Savage, James Goodwin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Antilymphocyte antibodies (ALA) in rheumatoid arthritis (RA) have increased reactivity with phytohemagglutinin (PHA) activated lymphoblasts which are known to have increased expression of Ia antigen. The present experiments suggest that part of this reactivity represents an Ia specificity of ALA. Fifteen of 18 RA sera tested were able to inhibit the binding of monoclonal anti-Ia antibodies as measured by a rosette method. RA sera did not inhibit the binding of other monoclonal antibodies: anti-OKT3, anti-OKT4, and anti-OKT8. The ability of RA sera to inhibit the binding of anti-Ia antibody was eliminated after absorption of the RA sera with an Ia positive human cell line (B35M) but not by an Ia negative line (MOLT4). Blocking of anti-Ia binding was greater in the IgG fraction of the RA sera but also occurred in the IgM fraction. Experiments including ultracentrifugation, pepsin digestion of RA sera, and preincubation of lymphoblasts with aggregated IgG demonstrated that Fc binding by RA sera was not a factor. Both monoclonal anti-Ia and anti-Ia hetero-antiserum also had increased reactivity with lymphoblast target cells. Pepsin digested Fab fragments of the anti-Ia heteroantiserum were able to block the activity of cytotoxic RA serum. However, ALA cytotoxic to lymphoblasts did not correlate with anti-Ia by rosette method. Ia-specific ALA by rosette method was associated with donor variability but did not appear to be HLA-DR restricted. Ia-specific ALA did not correlate with disease activity. These data suggest that anti-Ia activity is present in RA sera and may play an immunoregulatory role in this disease.

Original languageEnglish (US)
Pages (from-to)486-493
Number of pages8
JournalArthritis and Rheumatism
Volume26
Issue number4
StatePublished - 1983
Externally publishedYes

Fingerprint

Antilymphocyte Serum
Rheumatoid Arthritis
Serum
Pepsin A
Anti-Idiotypic Antibodies
Immunoglobulin G
Muromonab-CD3
Immunoglobulin Fab Fragments
Ultracentrifugation
Histocompatibility Antigens Class II
Phytohemagglutinins
HLA-DR Antigens
Immunoglobulin M
Immune Sera
Digestion
Monoclonal Antibodies
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Searles, R. P., Okudaira, K., Savage, S. M., & Goodwin, J. (1983). Ia specific antilymphocyte antibodies in rheumatoid arthritis. Arthritis and Rheumatism, 26(4), 486-493.

Ia specific antilymphocyte antibodies in rheumatoid arthritis. / Searles, R. P.; Okudaira, K.; Savage, S. M.; Goodwin, James.

In: Arthritis and Rheumatism, Vol. 26, No. 4, 1983, p. 486-493.

Research output: Contribution to journalArticle

Searles, RP, Okudaira, K, Savage, SM & Goodwin, J 1983, 'Ia specific antilymphocyte antibodies in rheumatoid arthritis', Arthritis and Rheumatism, vol. 26, no. 4, pp. 486-493.
Searles, R. P. ; Okudaira, K. ; Savage, S. M. ; Goodwin, James. / Ia specific antilymphocyte antibodies in rheumatoid arthritis. In: Arthritis and Rheumatism. 1983 ; Vol. 26, No. 4. pp. 486-493.
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