Identification and characterization of a HeLa nuclear protein that specifically binds to the trans-activation-response (TAR) element of human immunodeficiency virus

Robert A. Marciniak, Mariano A. Garcia-Blanco, Phillip A. Sharp

Research output: Contribution to journalArticle

148 Scopus citations

Abstract

Human immunodeficiency virus type 1 RNAs contain a sequence, trans-activation-response (TAR) element, which is required for tat protein-mediated trans-activation of viral gene expression. We have identified a nuclear protein from extracts of HeLa cells that binds to the TAR element RNA in a sequence-specific manner. The binding of this 68-kDa polypeptide was detected by UV cross-linking proteins to TAR element RNA transcribed in vitro. Competition experiments were performed by using a partially purified preparation of the protein to quantify the relative binding affinities of TAR element RNA mutants. The binding affinity of the TAR mutants paralleled the reported ability of those mutants to support tat trans-activation in vivo. We propose that this cellular protein moderates TAR activity in vivo.

Original languageEnglish (US)
Pages (from-to)3624-3628
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number9
DOIs
StatePublished - May 1990
Externally publishedYes

    Fingerprint

Keywords

  • RNA-binding proteins
  • UV cross-linking

ASJC Scopus subject areas

  • General

Cite this