Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions

Tapas Hazra, Yoke W. Kow, Zafar Hatahet, Barry Imhoff, Istvan Boldogh, Sanath K. Mokkapati, Sankar Mitra, Tadahide Izumi

Research output: Contribution to journalArticle

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Abstract

Two candidate human orthologs of Escherichia coli MutM/Nei were recently identified in the human genome database, and one of these, NEH1, was characterized earlier (Hazra, T. K., Izumi, T., Boldogh, I., Imhoff, B., Kow, Y. W., Jaruga, P., and Dizdaroglu, M. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 3523-3528). Here we report characterization of the second protein, originally named NEH2 and now renamed NEIL2 (Nei-like). The 37-kDa wild-type NEIL2 expressed in and purified from E. coli has DNA glycosylase/AP lyase activity, primarily for excising oxidative products of cytosine, with highest activity for 5-hydroxyuracil, one of the most abundant and mutagenic lesions induced by reactive oxygen species, and with lower activity for 5,6-dihydrouracil and 5-hydroxycytosine. It has negligible or undetectable activity with 8-oxoguanine, thymine glycol, 2-hydroxyadenine, hypoxanthine, and xanthine. NEIL2 is similar to NEIL1 in having N-terminal Pro as the active site. However, unlike NEIL1, its expression was independent of the cell cycle stage in fibroblasts, and its highest expression was observed in the testes and skeletal muscle. Despite the absence of a putative nuclear localization signal, NEIL2 was predominantly localized in the nucleus. These results suggest that NEIL2 is involved in global genome repair mainly for removing oxidative products of cytosine.

Original languageEnglish (US)
Pages (from-to)30417-30420
Number of pages4
JournalJournal of Biological Chemistry
Volume277
Issue number34
DOIs
StatePublished - Aug 23 2002

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DNA Glycosylases
Cytosine
DNA Repair
Escherichia coli
Repair
Genes
DNA-(Apurinic or Apyrimidinic Site) Lyase
Nuclear Localization Signals
Hypoxanthine
Xanthine
Human Genome
Fibroblasts
Muscle
Testis
Reactive Oxygen Species
Catalytic Domain
Cell Cycle
Skeletal Muscle
Cells
Genome

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions. / Hazra, Tapas; Kow, Yoke W.; Hatahet, Zafar; Imhoff, Barry; Boldogh, Istvan; Mokkapati, Sanath K.; Mitra, Sankar; Izumi, Tadahide.

In: Journal of Biological Chemistry, Vol. 277, No. 34, 23.08.2002, p. 30417-30420.

Research output: Contribution to journalArticle

Hazra, Tapas ; Kow, Yoke W. ; Hatahet, Zafar ; Imhoff, Barry ; Boldogh, Istvan ; Mokkapati, Sanath K. ; Mitra, Sankar ; Izumi, Tadahide. / Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 34. pp. 30417-30420.
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