Identification and characterization of "pathoadaptive mutations" of the cadBA operon in several intestinal Escherichia coli

Joerg Jores, Alfredo Torres, Sylke Wagner, Christopher B. Tutt, James B. Kaper, Lothar H. Wieler

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The dysenteric Shigella spp. and enteroinvasive Escherichia coli (EIEC) have evolved from commensal E. coli by the acquisition of a virulence plasmid and inactivation of genes of the cad locus encoding lysine decarboxylase (LDC) by so-called pathoadaptive mutation. As horizontal gene transfer and recombination occurs frequently in E. coli we were interested to see if similar pathoadaptive mutations are commonly present in other intestinal pathotypes. Therefore, we examined 140 intestinal E. coli strains of various pathotypes and the ECOR collection for their ability to decarboxylate lysine, and identified 25 strains that were unable to do so. Complementation of a Shiga toxin-producing E. coli and two enteropathogenic E. coli strains, both LDC-negative, with the intact cad locus restored LDC activity and resulted in a reduction in adherence to tissue culture cells. We investigated the cad locus for possible alterations by using hybridization and PCR techniques and compared the results with the alterations reported for Shigella spp. and EIEC strains. Interestingly, the alterations of the cad genes were similar to those previously reported, pointing towards a parallel evolution of LDC silencing in different intestinal E. coli pathotypes.

Original languageEnglish (US)
Pages (from-to)547-552
Number of pages6
JournalInternational Journal of Medical Microbiology
Volume296
Issue number8
DOIs
StatePublished - Dec 4 2006

Fingerprint

lysine decarboxylase
Operon
Escherichia coli
Mutation
Shigella
Shiga-Toxigenic Escherichia coli
Enteropathogenic Escherichia coli
Horizontal Gene Transfer
Gene Silencing
Genetic Recombination
Lysine
Virulence
Plasmids
Cell Culture Techniques
Polymerase Chain Reaction
Genes

Keywords

  • cadBA
  • E. coli
  • Lysine decarboxylase
  • Pathoadaptive mutation

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Identification and characterization of "pathoadaptive mutations" of the cadBA operon in several intestinal Escherichia coli. / Jores, Joerg; Torres, Alfredo; Wagner, Sylke; Tutt, Christopher B.; Kaper, James B.; Wieler, Lothar H.

In: International Journal of Medical Microbiology, Vol. 296, No. 8, 04.12.2006, p. 547-552.

Research output: Contribution to journalArticle

Jores, Joerg ; Torres, Alfredo ; Wagner, Sylke ; Tutt, Christopher B. ; Kaper, James B. ; Wieler, Lothar H. / Identification and characterization of "pathoadaptive mutations" of the cadBA operon in several intestinal Escherichia coli. In: International Journal of Medical Microbiology. 2006 ; Vol. 296, No. 8. pp. 547-552.
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