Identification and characterization of the SMT3 cDNA and gene from nematode Caenorhabditis elegans

Barun Choudhury, Steven S.L. Li

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

SMT3 gene from nematode, Caenorhabditis elegans, was identified and sequenced. The nematode SMT3 gene codes for a homolog to the yeast SMT3, suppressor of MIF2 mutation in a centromere protein gene. Further, nematode SMT3 cDNA was amplified by polymerase chain reaction from a cDNA library and its nucleotide sequence determined. A comparison of the SMT3 genomic and cDNA sequences established that the protein-encoding sequence is interrupted by two introns of 56 and 50 bp at codon Nos. 22-23 and 56, respectively. The sequence of 91 amino acids deduced from the nematode SMT3 nucleotide sequence exhibited 42 and 47% identity to the yeast and human SMT3 protein sequences, respectively. The evolutionary relationships of SMT3 proteins from human, nematode, Arabidopsis, rice, and yeast were analyzed.

Original languageEnglish (US)
Pages (from-to)788-791
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume234
Issue number3
DOIs
StatePublished - May 29 1997
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Yeast
Complementary DNA
Genes
Yeasts
Nucleotides
Genetic Suppression
Centromere
Polymerase chain reaction
Gene Library
Arabidopsis
Codon
Introns
Amino Acid Sequence
Proteins
Amino Acids
Polymerase Chain Reaction
human SUMO2 protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification and characterization of the SMT3 cDNA and gene from nematode Caenorhabditis elegans. / Choudhury, Barun; Li, Steven S.L.

In: Biochemical and Biophysical Research Communications, Vol. 234, No. 3, 29.05.1997, p. 788-791.

Research output: Contribution to journalArticle

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