Identification and enzymatic activities of four protein disulfide isomerase (PDI) isoforms of Leishmania amazonensis

B. X. Hong, Lynn Soong

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Leishmania parasites primarily infect cells of macrophage lineage and can cause leishmaniasis in the skin, mucosal, and visceral organs, depending on both host- and parasite-derived factors. The protein disulfide isomerases (PDIs) are thiol-disulfide oxidoreductases that catalyze the formation, reduction, and isomerization of disulfide bonds of proteins in cells. Although four Leishmania PDI genes are functionally inferred from homology in the genome sequences, only two of them have been expressed as active proteins to date. The functional relationship among various PDI enzymes remains largely unclear. In this study, we expressed and partially characterized all four L. amazonensis PDIs encoding 52-, 47-, 40-, and 15-kDa proteins. Homology analysis showed that the sequence identity between L. amazonensis (New World) PDIs and their counterpart PDI sequences from L. major (Old World) ranged from 76% to 99%. Kinetic characterization indicated that while the 15-, 40-, and 47- kDa PDI proteins displayed both insulin isomerase and reductase activities, the 52-kDa protein had only isomerase activity with no detectable reductase activity. All four PDI proteins were recognized by sera from L. amazonensis-infected mice and were sensitive to inhibition by standard PDI inhibitors. This study describes the enzymatic activities of recombinant L. amazonensis PDIs and suggests a role for these proteins in parasite development.

Original languageEnglish (US)
Pages (from-to)437-446
Number of pages10
JournalParasitology Research
Volume102
Issue number3
DOIs
StatePublished - Feb 2008

Fingerprint

Leishmania amazonensis
protein disulfide-isomerase
Protein Disulfide-Isomerases
Leishmania
Protein Isoforms
Proteins
proteins
Isomerases
Parasites
isomerases
parasites
Oxidoreductases
Protein Disulfide Reductase (Glutathione)
Leishmaniasis
leishmaniasis
isomerization
disulfide bonds
oxidoreductases
Cell Lineage
thiols

Keywords

  • Leishmania
  • Protein disulfide isomerase (PDI)

ASJC Scopus subject areas

  • Parasitology

Cite this

Identification and enzymatic activities of four protein disulfide isomerase (PDI) isoforms of Leishmania amazonensis. / Hong, B. X.; Soong, Lynn.

In: Parasitology Research, Vol. 102, No. 3, 02.2008, p. 437-446.

Research output: Contribution to journalArticle

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