Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera

Miridae)

Ping Ji, Shao Hua Gu, Jing Tao Liu, Xiao Qiang Zhu, Yu Yuan Guo, Jing Jiang Zhou, Yong Jun Zhang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Insect odorant-binding proteins (OBPs) are one group of olfactory-related proteins and are believed to play essential roles in the physiological activity of insects. In this study, we constructed a cDNA library from the antennae of Apolygus lucorum (Meyer-Dür). Twelve novel putative OBP genes were identified from the antennal cDNA library, and the expression profiles of the 12 OBPs in different tissues (antennae, heads, thoraxes, abdomens, legs and wings) were analyzed by real-time quantitative PCR. The results revealed that AlucOBP2, AlucOBP7, AlucOBP8, AlucOBP11 and AlucOBP12 are highly expressed in antennae. Especially the transcript levels of AlucOBP7, AlucOBP8 and AlucOBP11 in male antennae were significantly higher than in female antennae, suggesting their potential olfactory functions in host seeking and mate finding of A. lucorum. However, AlucOBP1, AlucOBP5 and AlucOBP6 were highly expressed in the heads, whereas AlucOBP3 was specifically expressed only in the legs. In addition, AlucOBP4, AlucOBP9 and AlucOBP10 were expressed in almost all chemosensory tissues (antennae, heads, wings and legs). Our research is meaningful for understanding the molecular basis of the insect chemoreception requirements and provides valuable target genes to control the insect pests.

Original languageEnglish (US)
Pages (from-to)301-311
Number of pages11
JournalApplied Entomology and Zoology
Volume48
Issue number3
DOIs
StatePublished - Aug 1 2013
Externally publishedYes

Fingerprint

Miridae
antennae
Hemiptera
legs
genes
cDNA libraries
insect proteins
host seeking
insects
abdomen
insect pests
odorant receptors
quantitative polymerase chain reaction
proteins

Keywords

  • Antennal cDNA library
  • Apolygus lucorum
  • Expression profiles
  • Odorant-binding proteins
  • Olfaction

ASJC Scopus subject areas

  • Insect Science

Cite this

Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera : Miridae). / Ji, Ping; Gu, Shao Hua; Liu, Jing Tao; Zhu, Xiao Qiang; Guo, Yu Yuan; Zhou, Jing Jiang; Zhang, Yong Jun.

In: Applied Entomology and Zoology, Vol. 48, No. 3, 01.08.2013, p. 301-311.

Research output: Contribution to journalArticle

Ji, Ping ; Gu, Shao Hua ; Liu, Jing Tao ; Zhu, Xiao Qiang ; Guo, Yu Yuan ; Zhou, Jing Jiang ; Zhang, Yong Jun. / Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera : Miridae). In: Applied Entomology and Zoology. 2013 ; Vol. 48, No. 3. pp. 301-311.
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