Identification and functional analysis of an immunoreactive DsbA-like thio-disulfide oxidoreductase of Ehrlichia spp.

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Novel homologous DsbA-like disulfide bond formation (Dsb) proteins of Ehrlichia chaffeensis and Ehrlichia canis were identified which restored DsbA activity in complemented Escherichia coli dsbA mutants. Recombinant Ehrlichia Dsb (eDsb) proteins were recognized by sera from E. canis-infected dogs but not from E. chaffeensis-infected patients. The eDsb proteins were observed primarily in the periplasm of E. chaffeensis and E. canis.

Original languageEnglish (US)
Pages (from-to)2700-2703
Number of pages4
JournalInfection and Immunity
Volume70
Issue number5
DOIs
StatePublished - 2002

Fingerprint

Ehrlichia chaffeensis
Ehrlichia canis
Ehrlichia
Disulfides
Oxidoreductases
Periplasm
Proteins
Dogs
Escherichia coli
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

@article{0d0c90c2fd4c4900b21f24ae0a0ed3fc,
title = "Identification and functional analysis of an immunoreactive DsbA-like thio-disulfide oxidoreductase of Ehrlichia spp.",
abstract = "Novel homologous DsbA-like disulfide bond formation (Dsb) proteins of Ehrlichia chaffeensis and Ehrlichia canis were identified which restored DsbA activity in complemented Escherichia coli dsbA mutants. Recombinant Ehrlichia Dsb (eDsb) proteins were recognized by sera from E. canis-infected dogs but not from E. chaffeensis-infected patients. The eDsb proteins were observed primarily in the periplasm of E. chaffeensis and E. canis.",
author = "Jere McBride and Ndip, {Lucy M.} and Vsevolod Popov and David Walker",
year = "2002",
doi = "10.1128/IAI.70.5.2700-2703.2002",
language = "English (US)",
volume = "70",
pages = "2700--2703",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Identification and functional analysis of an immunoreactive DsbA-like thio-disulfide oxidoreductase of Ehrlichia spp.

AU - McBride, Jere

AU - Ndip, Lucy M.

AU - Popov, Vsevolod

AU - Walker, David

PY - 2002

Y1 - 2002

N2 - Novel homologous DsbA-like disulfide bond formation (Dsb) proteins of Ehrlichia chaffeensis and Ehrlichia canis were identified which restored DsbA activity in complemented Escherichia coli dsbA mutants. Recombinant Ehrlichia Dsb (eDsb) proteins were recognized by sera from E. canis-infected dogs but not from E. chaffeensis-infected patients. The eDsb proteins were observed primarily in the periplasm of E. chaffeensis and E. canis.

AB - Novel homologous DsbA-like disulfide bond formation (Dsb) proteins of Ehrlichia chaffeensis and Ehrlichia canis were identified which restored DsbA activity in complemented Escherichia coli dsbA mutants. Recombinant Ehrlichia Dsb (eDsb) proteins were recognized by sera from E. canis-infected dogs but not from E. chaffeensis-infected patients. The eDsb proteins were observed primarily in the periplasm of E. chaffeensis and E. canis.

UR - http://www.scopus.com/inward/record.url?scp=0036117915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036117915&partnerID=8YFLogxK

U2 - 10.1128/IAI.70.5.2700-2703.2002

DO - 10.1128/IAI.70.5.2700-2703.2002

M3 - Article

C2 - 11953415

AN - SCOPUS:0036117915

VL - 70

SP - 2700

EP - 2703

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -