Identification and functional characterization of tRNA-derived RNA fragments (tRFs) in respiratory syncytial virus infection

Qingrong Wang, Inhan Lee, Junping Ren, Subramanian Shankar Ajay, Yong Sun Lee, Xiaoyong Bao

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

The discovery of small noncoding RNAs (sncRNAs) with regulatory functions is a recent breakthrough in biology. Among sncRNAs, microRNA (miRNA), derived from host or virus, has emerged as elements with high importance in control of viral replication and host responses. However, the expression pattern and functional aspects of other types of sncRNAs, following viral infection, are unexplored. In order to define expression patterns of sncRNAs, as well as to discover novel regulatory sncRNAs in response to viral infection, we applied deep sequencing to cells infected with human respiratory syncytial virus (RSV), the most common cause of bronchiolitis and pneumonia in babies. RSV infection leads to abundant production of transfer RNA (tRNA)-derived RNA Fragments (tRFs) that are ∼30 nucleotides (nts) long and correspond to the 5′-half of mature tRNAs. At least one tRF, which is derived from tRNA-Glu-CTC, represses target mRNA in the cytoplasm and promotes RSV replication. This demonstrates that this tRF is not a random by-product of tRNA degradation but a functional molecule. The biogenesis of this tRF is also specific, as it is mediated by the endonuclease angiogenin (ANG), not by other nucleases. In summary, our study presents novel information on the induction of a functional tRF by viral infection.

Original languageEnglish (US)
Pages (from-to)368-379
Number of pages12
JournalMolecular Therapy
Volume21
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Respiratory Syncytial Virus Infections
Small Untranslated RNA
Transfer RNA
RNA
Virus Diseases
RNA, Transfer, Glu
Human respiratory syncytial virus
High-Throughput Nucleotide Sequencing
Bronchiolitis
Respiratory Syncytial Viruses
Endonucleases
RNA Stability
Virus Replication
MicroRNAs
Pneumonia
Cytoplasm
Nucleotides
Viruses
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Genetics
  • Drug Discovery
  • Pharmacology

Cite this

Identification and functional characterization of tRNA-derived RNA fragments (tRFs) in respiratory syncytial virus infection. / Wang, Qingrong; Lee, Inhan; Ren, Junping; Ajay, Subramanian Shankar; Lee, Yong Sun; Bao, Xiaoyong.

In: Molecular Therapy, Vol. 21, No. 2, 02.2013, p. 368-379.

Research output: Contribution to journalArticle

Wang, Qingrong ; Lee, Inhan ; Ren, Junping ; Ajay, Subramanian Shankar ; Lee, Yong Sun ; Bao, Xiaoyong. / Identification and functional characterization of tRNA-derived RNA fragments (tRFs) in respiratory syncytial virus infection. In: Molecular Therapy. 2013 ; Vol. 21, No. 2. pp. 368-379.
@article{4102f43b89404af49b3995ae10a6742c,
title = "Identification and functional characterization of tRNA-derived RNA fragments (tRFs) in respiratory syncytial virus infection",
abstract = "The discovery of small noncoding RNAs (sncRNAs) with regulatory functions is a recent breakthrough in biology. Among sncRNAs, microRNA (miRNA), derived from host or virus, has emerged as elements with high importance in control of viral replication and host responses. However, the expression pattern and functional aspects of other types of sncRNAs, following viral infection, are unexplored. In order to define expression patterns of sncRNAs, as well as to discover novel regulatory sncRNAs in response to viral infection, we applied deep sequencing to cells infected with human respiratory syncytial virus (RSV), the most common cause of bronchiolitis and pneumonia in babies. RSV infection leads to abundant production of transfer RNA (tRNA)-derived RNA Fragments (tRFs) that are ∼30 nucleotides (nts) long and correspond to the 5′-half of mature tRNAs. At least one tRF, which is derived from tRNA-Glu-CTC, represses target mRNA in the cytoplasm and promotes RSV replication. This demonstrates that this tRF is not a random by-product of tRNA degradation but a functional molecule. The biogenesis of this tRF is also specific, as it is mediated by the endonuclease angiogenin (ANG), not by other nucleases. In summary, our study presents novel information on the induction of a functional tRF by viral infection.",
author = "Qingrong Wang and Inhan Lee and Junping Ren and Ajay, {Subramanian Shankar} and Lee, {Yong Sun} and Xiaoyong Bao",
year = "2013",
month = "2",
doi = "10.1038/mt.2012.237",
language = "English (US)",
volume = "21",
pages = "368--379",
journal = "Molecular Therapy",
issn = "1525-0016",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Identification and functional characterization of tRNA-derived RNA fragments (tRFs) in respiratory syncytial virus infection

AU - Wang, Qingrong

AU - Lee, Inhan

AU - Ren, Junping

AU - Ajay, Subramanian Shankar

AU - Lee, Yong Sun

AU - Bao, Xiaoyong

PY - 2013/2

Y1 - 2013/2

N2 - The discovery of small noncoding RNAs (sncRNAs) with regulatory functions is a recent breakthrough in biology. Among sncRNAs, microRNA (miRNA), derived from host or virus, has emerged as elements with high importance in control of viral replication and host responses. However, the expression pattern and functional aspects of other types of sncRNAs, following viral infection, are unexplored. In order to define expression patterns of sncRNAs, as well as to discover novel regulatory sncRNAs in response to viral infection, we applied deep sequencing to cells infected with human respiratory syncytial virus (RSV), the most common cause of bronchiolitis and pneumonia in babies. RSV infection leads to abundant production of transfer RNA (tRNA)-derived RNA Fragments (tRFs) that are ∼30 nucleotides (nts) long and correspond to the 5′-half of mature tRNAs. At least one tRF, which is derived from tRNA-Glu-CTC, represses target mRNA in the cytoplasm and promotes RSV replication. This demonstrates that this tRF is not a random by-product of tRNA degradation but a functional molecule. The biogenesis of this tRF is also specific, as it is mediated by the endonuclease angiogenin (ANG), not by other nucleases. In summary, our study presents novel information on the induction of a functional tRF by viral infection.

AB - The discovery of small noncoding RNAs (sncRNAs) with regulatory functions is a recent breakthrough in biology. Among sncRNAs, microRNA (miRNA), derived from host or virus, has emerged as elements with high importance in control of viral replication and host responses. However, the expression pattern and functional aspects of other types of sncRNAs, following viral infection, are unexplored. In order to define expression patterns of sncRNAs, as well as to discover novel regulatory sncRNAs in response to viral infection, we applied deep sequencing to cells infected with human respiratory syncytial virus (RSV), the most common cause of bronchiolitis and pneumonia in babies. RSV infection leads to abundant production of transfer RNA (tRNA)-derived RNA Fragments (tRFs) that are ∼30 nucleotides (nts) long and correspond to the 5′-half of mature tRNAs. At least one tRF, which is derived from tRNA-Glu-CTC, represses target mRNA in the cytoplasm and promotes RSV replication. This demonstrates that this tRF is not a random by-product of tRNA degradation but a functional molecule. The biogenesis of this tRF is also specific, as it is mediated by the endonuclease angiogenin (ANG), not by other nucleases. In summary, our study presents novel information on the induction of a functional tRF by viral infection.

UR - http://www.scopus.com/inward/record.url?scp=84873409537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873409537&partnerID=8YFLogxK

U2 - 10.1038/mt.2012.237

DO - 10.1038/mt.2012.237

M3 - Article

VL - 21

SP - 368

EP - 379

JO - Molecular Therapy

JF - Molecular Therapy

SN - 1525-0016

IS - 2

ER -