Identification and genetic analysis of Panama-genotype Venezuelan equine encephalitis virus subtype ID in Peru

M. Steven Oberste, Scott Weaver, Douglas M. Watts, Jonathan F. Smith

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Venezuelan equine encephalitis (VEE) virus was isolated in 1993, 1994, and 1995 from human cases of acute, undifferentiated, febrile illness in the Peruvian Amazon Basin. Two virus isolates were recovered in 1994 from Peruvian soldiers at a jungle outpost near Pantoja in northern Peru, and 10 isolates were obtained from military personnel and civilians in 1993-1995 in Iquitos, an urban center in northeastern Peru. The genetic relationship of these isolates to other VEE virus strains was determined by sequencing 856- 867 nucleotide reverse transcription-polymerase chain reaction fragments derived from the PE2 glycoprotein gene. The sequences were compared with those of other VEE virus strains, including representatives of the LAB, IC, ID, IE, II, and IIIC subtypes. The two Pantoja isolates were most closely related to subtype IC and ID viruses previously isolated in Colombia and Venezuela, and to the ID viruses isolated during the 1970s in Iquitos. All of the recent Iquitos isolates were similar to one another, but they were more closely related to Panamanian ID strains than to isolates previously obtained in Iquitos, Peru, or in Colombia and Venezuela. The recent Iquitos VEE viral isolates were the first Panama-genotype VEE ID virus strains identified outside of the Republic of Panama.

Original languageEnglish (US)
Pages (from-to)41-46
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume58
Issue number1
StatePublished - 1998

Fingerprint

Venezuelan Equine Encephalitis Viruses
Panama
Peru
Genotype
Venezuela
Colombia
Military Personnel
Viruses
Venezuelan Equine Encephalomyelitides
Reverse Transcription
Glycoproteins
Fever
Nucleotides
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Identification and genetic analysis of Panama-genotype Venezuelan equine encephalitis virus subtype ID in Peru. / Oberste, M. Steven; Weaver, Scott; Watts, Douglas M.; Smith, Jonathan F.

In: American Journal of Tropical Medicine and Hygiene, Vol. 58, No. 1, 1998, p. 41-46.

Research output: Contribution to journalArticle

@article{0b7ab8fea2e64b11b14d21aca62c9fae,
title = "Identification and genetic analysis of Panama-genotype Venezuelan equine encephalitis virus subtype ID in Peru",
abstract = "Venezuelan equine encephalitis (VEE) virus was isolated in 1993, 1994, and 1995 from human cases of acute, undifferentiated, febrile illness in the Peruvian Amazon Basin. Two virus isolates were recovered in 1994 from Peruvian soldiers at a jungle outpost near Pantoja in northern Peru, and 10 isolates were obtained from military personnel and civilians in 1993-1995 in Iquitos, an urban center in northeastern Peru. The genetic relationship of these isolates to other VEE virus strains was determined by sequencing 856- 867 nucleotide reverse transcription-polymerase chain reaction fragments derived from the PE2 glycoprotein gene. The sequences were compared with those of other VEE virus strains, including representatives of the LAB, IC, ID, IE, II, and IIIC subtypes. The two Pantoja isolates were most closely related to subtype IC and ID viruses previously isolated in Colombia and Venezuela, and to the ID viruses isolated during the 1970s in Iquitos. All of the recent Iquitos isolates were similar to one another, but they were more closely related to Panamanian ID strains than to isolates previously obtained in Iquitos, Peru, or in Colombia and Venezuela. The recent Iquitos VEE viral isolates were the first Panama-genotype VEE ID virus strains identified outside of the Republic of Panama.",
author = "Oberste, {M. Steven} and Scott Weaver and Watts, {Douglas M.} and Smith, {Jonathan F.}",
year = "1998",
language = "English (US)",
volume = "58",
pages = "41--46",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "1",

}

TY - JOUR

T1 - Identification and genetic analysis of Panama-genotype Venezuelan equine encephalitis virus subtype ID in Peru

AU - Oberste, M. Steven

AU - Weaver, Scott

AU - Watts, Douglas M.

AU - Smith, Jonathan F.

PY - 1998

Y1 - 1998

N2 - Venezuelan equine encephalitis (VEE) virus was isolated in 1993, 1994, and 1995 from human cases of acute, undifferentiated, febrile illness in the Peruvian Amazon Basin. Two virus isolates were recovered in 1994 from Peruvian soldiers at a jungle outpost near Pantoja in northern Peru, and 10 isolates were obtained from military personnel and civilians in 1993-1995 in Iquitos, an urban center in northeastern Peru. The genetic relationship of these isolates to other VEE virus strains was determined by sequencing 856- 867 nucleotide reverse transcription-polymerase chain reaction fragments derived from the PE2 glycoprotein gene. The sequences were compared with those of other VEE virus strains, including representatives of the LAB, IC, ID, IE, II, and IIIC subtypes. The two Pantoja isolates were most closely related to subtype IC and ID viruses previously isolated in Colombia and Venezuela, and to the ID viruses isolated during the 1970s in Iquitos. All of the recent Iquitos isolates were similar to one another, but they were more closely related to Panamanian ID strains than to isolates previously obtained in Iquitos, Peru, or in Colombia and Venezuela. The recent Iquitos VEE viral isolates were the first Panama-genotype VEE ID virus strains identified outside of the Republic of Panama.

AB - Venezuelan equine encephalitis (VEE) virus was isolated in 1993, 1994, and 1995 from human cases of acute, undifferentiated, febrile illness in the Peruvian Amazon Basin. Two virus isolates were recovered in 1994 from Peruvian soldiers at a jungle outpost near Pantoja in northern Peru, and 10 isolates were obtained from military personnel and civilians in 1993-1995 in Iquitos, an urban center in northeastern Peru. The genetic relationship of these isolates to other VEE virus strains was determined by sequencing 856- 867 nucleotide reverse transcription-polymerase chain reaction fragments derived from the PE2 glycoprotein gene. The sequences were compared with those of other VEE virus strains, including representatives of the LAB, IC, ID, IE, II, and IIIC subtypes. The two Pantoja isolates were most closely related to subtype IC and ID viruses previously isolated in Colombia and Venezuela, and to the ID viruses isolated during the 1970s in Iquitos. All of the recent Iquitos isolates were similar to one another, but they were more closely related to Panamanian ID strains than to isolates previously obtained in Iquitos, Peru, or in Colombia and Venezuela. The recent Iquitos VEE viral isolates were the first Panama-genotype VEE ID virus strains identified outside of the Republic of Panama.

UR - http://www.scopus.com/inward/record.url?scp=0031943796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031943796&partnerID=8YFLogxK

M3 - Article

C2 - 9452290

AN - SCOPUS:0031943796

VL - 58

SP - 41

EP - 46

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 1

ER -