Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns

Mariano Garcia-Blanco, S. F. Jamison, P. A. Sharp

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

A protein of molecular size 62,000 daltons (p62) was detected in HeLa cell nuclear extracts by UV cross-linking to mRNA precursors. p62 binds specifically to the polypyrimidine tract of the 3' splice site region of introns. p62 purified to homogeneity binds the polypyrimidine tract of pre-mRNAs. This binding does not require the AG dinucleotide at the 3' splice site. Alterations in the polypyrimidine tract that reduce the binding of p62 yield a corresponding reduction in the efficiency of formation of a U2 snRNP/pre-mRNA complex and splicing. The p62 protein is retained in the spliceosome, where it remains bound to the pre-mRNA. This polypyrimidine tract binding protein (pPTB) is proposed to be a critical component in recognition of the 3' splice site during splicing.

Original languageEnglish (US)
Pages (from-to)1874-1886
Number of pages13
JournalGenes and Development
Volume3
Issue number12 A
StatePublished - 1989
Externally publishedYes

Fingerprint

RNA Precursors
Introns
RNA Splice Sites
Proteins
U2 Small Nuclear Ribonucleoproteins
Polypyrimidine Tract-Binding Protein
Spliceosomes
Cell Extracts
HeLa Cells

Keywords

  • 3' splice site
  • intron
  • Polypyrimidine tract
  • RNA-binding protein
  • UV cross-linking

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. / Garcia-Blanco, Mariano; Jamison, S. F.; Sharp, P. A.

In: Genes and Development, Vol. 3, No. 12 A, 1989, p. 1874-1886.

Research output: Contribution to journalArticle

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