Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns

M. A. Garcia-Blanco, S. F. Jamison, P. A. Sharp

Research output: Contribution to journalArticle

230 Scopus citations

Abstract

A protein of molecular size 62,000 daltons (p62) was detected in HeLa cell nuclear extracts by UV cross-linking to mRNA precursors. p62 binds specifically to the polypyrimidine tract of the 3' splice site region of introns. p62 purified to homogeneity binds the polypyrimidine tract of pre-mRNAs. This binding does not require the AG dinucleotide at the 3' splice site. Alterations in the polypyrimidine tract that reduce the binding of p62 yield a corresponding reduction in the efficiency of formation of a U2 snRNP/pre-mRNA complex and splicing. The p62 protein is retained in the spliceosome, where it remains bound to the pre-mRNA. This polypyrimidine tract binding protein (pPTB) is proposed to be a critical component in recognition of the 3' splice site during splicing.

Original languageEnglish (US)
Pages (from-to)1874-1886
Number of pages13
JournalGenes and Development
Volume3
Issue number12 A
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Keywords

  • 3' splice site
  • Polypyrimidine tract
  • RNA-binding protein
  • UV cross-linking
  • intron

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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