Identification and purification of a novel G protein from neutrophils

Burton F. Dickey, Hae Yung Pyun, Kim C. Williamson, Javier Navarro

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A novel G protein which appears to couple chemotactic peptide receptors to a polyphosphoinositide phospholipase C has been purified from rabbit neutrophils. Neutrophil membranes were solubilized with sodium cholate and fractionated by successive anion exchange, gel filtration and hydrophobic chromatography. Guanosine-5′-(3-O-thio)triphosphate binding activity was purified 170-fold from the soluble extract. The α-subunit of the purified G protein was identified by pertussis toxin-catalyzed ADP-ribosylation, and found to have an Mr of 40 000. The β-subunit (Mr 36 000) comigrated on SDS-polyacrylamide gel electrophoresis with the β-subunits of bovine brain Gi and Go. The neutrophil pertussis toxin substrate is highly unstable in cholate solution unless 30% ethylene glycol is added. Structural and functional analysis of this novel G protein will advance our understanding of the molecular mechanisms of coupling of receptors to phospholipase C.

Original languageEnglish (US)
Pages (from-to)289-292
Number of pages4
JournalFEBS Letters
Volume219
Issue number2
DOIs
StatePublished - Jul 27 1987
Externally publishedYes

Fingerprint

GTP-Binding Proteins
Purification
Neutrophils
Pertussis Toxin
Hydrophobic chromatography
Sodium Cholate
Formyl Peptide Receptor
Cholates
Guanosine 5'-O-(3-Thiotriphosphate)
Phosphoinositide Phospholipase C
Functional analysis
Ethylene Glycol
Type C Phospholipases
Electrophoresis
Structural analysis
Adenosine Diphosphate
Gel Chromatography
Anions
Polyacrylamide Gel Electrophoresis
Brain

Keywords

  • (Rabbit neutrophil)
  • fMet-Leu-Phe receptor
  • Guanine nucleotide-binding protein
  • Phospholipase C

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification and purification of a novel G protein from neutrophils. / Dickey, Burton F.; Pyun, Hae Yung; Williamson, Kim C.; Navarro, Javier.

In: FEBS Letters, Vol. 219, No. 2, 27.07.1987, p. 289-292.

Research output: Contribution to journalArticle

Dickey, Burton F. ; Pyun, Hae Yung ; Williamson, Kim C. ; Navarro, Javier. / Identification and purification of a novel G protein from neutrophils. In: FEBS Letters. 1987 ; Vol. 219, No. 2. pp. 289-292.
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