Identification and quantification of human brown adipose tissue

Maria Chondronikola, Craig Porter, John Ogunbileje, Labros S. Sidossis

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Brown adipose tissue (BAT) has attracted significant interest as a potential target tissue against obesity and its associated metabolic perturbations. The purpose of this chapter is to provide an overview of some of the methodological approaches that can be used to identify and quantify BAT in people. Specifically, we will provide a step-by-step description of the following procedures: quantification of BAT in vivo using positron emission tomography-computed tomography (PET/CT) with 2-deoxy-2-[18F]fluoroglucose (18F-FDG) as a tracer, mitochondrial respiration, and uncoupling protein 1 (UCP1) gene and protein expression.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages159-176
Number of pages18
Volume1566
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1566
ISSN (Print)10643745

Fingerprint

Forensic Anthropology
Brown Adipose Tissue
Fluorodeoxyglucose F18
Respiration
Obesity
Gene Expression
Proteins

Keywords

  • 2-deoxy-2-[F]fluoroglucose
  • Biopsy
  • Brown adipose tissue
  • Positron emission tomography-computed tomography
  • Uncoupling protein 1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chondronikola, M., Porter, C., Ogunbileje, J., & Sidossis, L. S. (2017). Identification and quantification of human brown adipose tissue. In Methods in Molecular Biology (Vol. 1566, pp. 159-176). (Methods in Molecular Biology; Vol. 1566). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6820-6_16

Identification and quantification of human brown adipose tissue. / Chondronikola, Maria; Porter, Craig; Ogunbileje, John; Sidossis, Labros S.

Methods in Molecular Biology. Vol. 1566 Humana Press Inc., 2017. p. 159-176 (Methods in Molecular Biology; Vol. 1566).

Research output: Chapter in Book/Report/Conference proceedingChapter

Chondronikola, M, Porter, C, Ogunbileje, J & Sidossis, LS 2017, Identification and quantification of human brown adipose tissue. in Methods in Molecular Biology. vol. 1566, Methods in Molecular Biology, vol. 1566, Humana Press Inc., pp. 159-176. https://doi.org/10.1007/978-1-4939-6820-6_16
Chondronikola M, Porter C, Ogunbileje J, Sidossis LS. Identification and quantification of human brown adipose tissue. In Methods in Molecular Biology. Vol. 1566. Humana Press Inc. 2017. p. 159-176. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6820-6_16
Chondronikola, Maria ; Porter, Craig ; Ogunbileje, John ; Sidossis, Labros S. / Identification and quantification of human brown adipose tissue. Methods in Molecular Biology. Vol. 1566 Humana Press Inc., 2017. pp. 159-176 (Methods in Molecular Biology).
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