Identification and quantification of human brown adipose tissue

Maria Chondronikola, Craig Porter, John Ogunbileje, Labros S. Sidossis

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Brown adipose tissue (BAT) has attracted significant interest as a potential target tissue against obesity and its associated metabolic perturbations. The purpose of this chapter is to provide an overview of some of the methodological approaches that can be used to identify and quantify BAT in people. Specifically, we will provide a step-by-step description of the following procedures: quantification of BAT in vivo using positron emission tomography-computed tomography (PET/CT) with 2-deoxy-2-[18F]fluoroglucose (18F-FDG) as a tracer, mitochondrial respiration, and uncoupling protein 1 (UCP1) gene and protein expression.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages159-176
Number of pages18
Volume1566
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1566
ISSN (Print)10643745

Keywords

  • 2-deoxy-2-[F]fluoroglucose
  • Biopsy
  • Brown adipose tissue
  • Positron emission tomography-computed tomography
  • Uncoupling protein 1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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