Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

Hongjun Shu, Yingxin Zhao, Ge Zhou, She Chen, Julia D. Wulfkuhle, Dennis C. Sgroi, Henry Kruztsch, Kelly McLean, Melodie Knowlton, Aysegul Sahin, Yue Chen, Emanuel Petricoin, Patricia S. Steeg, Yingming Zhao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages227-228
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Microdissection
Cells
Annexins
Proteins
Tubulin
Electrophoresis
Mass spectrometry
Gels
Lasers
Epithelial Cells
fascin
Proteomics
Immunohistochemistry

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Shu, H., Zhao, Y., Zhou, G., Chen, S., Wulfkuhle, J. D., Sgroi, D. C., ... Zhao, Y. (2002). Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 227-228)

Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). / Shu, Hongjun; Zhao, Yingxin; Zhou, Ge; Chen, She; Wulfkuhle, Julia D.; Sgroi, Dennis C.; Kruztsch, Henry; McLean, Kelly; Knowlton, Melodie; Sahin, Aysegul; Chen, Yue; Petricoin, Emanuel; Steeg, Patricia S.; Zhao, Yingming.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 227-228.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shu, H, Zhao, Y, Zhou, G, Chen, S, Wulfkuhle, JD, Sgroi, DC, Kruztsch, H, McLean, K, Knowlton, M, Sahin, A, Chen, Y, Petricoin, E, Steeg, PS & Zhao, Y 2002, Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 227-228, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Shu H, Zhao Y, Zhou G, Chen S, Wulfkuhle JD, Sgroi DC et al. Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 227-228
Shu, Hongjun ; Zhao, Yingxin ; Zhou, Ge ; Chen, She ; Wulfkuhle, Julia D. ; Sgroi, Dennis C. ; Kruztsch, Henry ; McLean, Kelly ; Knowlton, Melodie ; Sahin, Aysegul ; Chen, Yue ; Petricoin, Emanuel ; Steeg, Patricia S. ; Zhao, Yingming. / Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 227-228
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abstract = "A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.",
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