Identification, Localization, and Quantification of HIV Reservoirs Using Microscopy

Lisa Prevedel, Nancy Ruel, Paul Castellano, Carla Smith, Shaily Malik, Courtney Villeux, Morgane Bomsel, Susan Morgello, Eliseo Eugenin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The major barrier to eradicating human immunodeficiency virus-1 (HIV) infection is the generation and extended survival of HIV reservoirs. In order to eradicate HIV infection, it is essential to detect, quantify, and characterize circulating and tissue-associated viral reservoirs in infected individuals. Currently, PCR-based technologies and Quantitative Viral Outgrowth Assays (Q-VOA) are the gold standards to detect viral reservoirs. However, these methods are limited to detecting circulating viral reservoirs, and it has been shown that they misrepresent the size of the reservoirs, largely because they detect only one component of the HIV life cycle and are unable to detect viral reservoirs in tissues. Here, we described the use of multiple detection systems to identify integrated HIV DNA or viral mRNA and several HIV proteins in circulating and tissue reservoirs using improved staining and microscopy techniques. We believe that this imaging-based approach for detecting HIV reservoirs will lead to breakthroughs necessary to eradicate these reservoirs.

Original languageEnglish (US)
Article numbere64
JournalCurrent Protocols in Cell Biology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

HIV-1
Microscopy
Virus Diseases
Human Immunodeficiency Virus Proteins
Viral DNA
Life Cycle Stages
Staining and Labeling
Technology
Polymerase Chain Reaction
Messenger RNA

Keywords

  • AIDS
  • astrocytes
  • macrophages
  • Q-VOA
  • T cells
  • transmission

ASJC Scopus subject areas

  • Cell Biology

Cite this

Identification, Localization, and Quantification of HIV Reservoirs Using Microscopy. / Prevedel, Lisa; Ruel, Nancy; Castellano, Paul; Smith, Carla; Malik, Shaily; Villeux, Courtney; Bomsel, Morgane; Morgello, Susan; Eugenin, Eliseo.

In: Current Protocols in Cell Biology, 01.01.2018.

Research output: Contribution to journalArticle

Prevedel, Lisa ; Ruel, Nancy ; Castellano, Paul ; Smith, Carla ; Malik, Shaily ; Villeux, Courtney ; Bomsel, Morgane ; Morgello, Susan ; Eugenin, Eliseo. / Identification, Localization, and Quantification of HIV Reservoirs Using Microscopy. In: Current Protocols in Cell Biology. 2018.
@article{045fed1f67ab47adaf9cda645d362dfd,
title = "Identification, Localization, and Quantification of HIV Reservoirs Using Microscopy",
abstract = "The major barrier to eradicating human immunodeficiency virus-1 (HIV) infection is the generation and extended survival of HIV reservoirs. In order to eradicate HIV infection, it is essential to detect, quantify, and characterize circulating and tissue-associated viral reservoirs in infected individuals. Currently, PCR-based technologies and Quantitative Viral Outgrowth Assays (Q-VOA) are the gold standards to detect viral reservoirs. However, these methods are limited to detecting circulating viral reservoirs, and it has been shown that they misrepresent the size of the reservoirs, largely because they detect only one component of the HIV life cycle and are unable to detect viral reservoirs in tissues. Here, we described the use of multiple detection systems to identify integrated HIV DNA or viral mRNA and several HIV proteins in circulating and tissue reservoirs using improved staining and microscopy techniques. We believe that this imaging-based approach for detecting HIV reservoirs will lead to breakthroughs necessary to eradicate these reservoirs.",
keywords = "AIDS, astrocytes, macrophages, Q-VOA, T cells, transmission",
author = "Lisa Prevedel and Nancy Ruel and Paul Castellano and Carla Smith and Shaily Malik and Courtney Villeux and Morgane Bomsel and Susan Morgello and Eliseo Eugenin",
year = "2018",
month = "1",
day = "1",
doi = "10.1002/cpcb.64",
language = "English (US)",
journal = "Current Protocols in Cell Biology",
issn = "1934-2500",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Identification, Localization, and Quantification of HIV Reservoirs Using Microscopy

AU - Prevedel, Lisa

AU - Ruel, Nancy

AU - Castellano, Paul

AU - Smith, Carla

AU - Malik, Shaily

AU - Villeux, Courtney

AU - Bomsel, Morgane

AU - Morgello, Susan

AU - Eugenin, Eliseo

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The major barrier to eradicating human immunodeficiency virus-1 (HIV) infection is the generation and extended survival of HIV reservoirs. In order to eradicate HIV infection, it is essential to detect, quantify, and characterize circulating and tissue-associated viral reservoirs in infected individuals. Currently, PCR-based technologies and Quantitative Viral Outgrowth Assays (Q-VOA) are the gold standards to detect viral reservoirs. However, these methods are limited to detecting circulating viral reservoirs, and it has been shown that they misrepresent the size of the reservoirs, largely because they detect only one component of the HIV life cycle and are unable to detect viral reservoirs in tissues. Here, we described the use of multiple detection systems to identify integrated HIV DNA or viral mRNA and several HIV proteins in circulating and tissue reservoirs using improved staining and microscopy techniques. We believe that this imaging-based approach for detecting HIV reservoirs will lead to breakthroughs necessary to eradicate these reservoirs.

AB - The major barrier to eradicating human immunodeficiency virus-1 (HIV) infection is the generation and extended survival of HIV reservoirs. In order to eradicate HIV infection, it is essential to detect, quantify, and characterize circulating and tissue-associated viral reservoirs in infected individuals. Currently, PCR-based technologies and Quantitative Viral Outgrowth Assays (Q-VOA) are the gold standards to detect viral reservoirs. However, these methods are limited to detecting circulating viral reservoirs, and it has been shown that they misrepresent the size of the reservoirs, largely because they detect only one component of the HIV life cycle and are unable to detect viral reservoirs in tissues. Here, we described the use of multiple detection systems to identify integrated HIV DNA or viral mRNA and several HIV proteins in circulating and tissue reservoirs using improved staining and microscopy techniques. We believe that this imaging-based approach for detecting HIV reservoirs will lead to breakthroughs necessary to eradicate these reservoirs.

KW - AIDS

KW - astrocytes

KW - macrophages

KW - Q-VOA

KW - T cells

KW - transmission

UR - http://www.scopus.com/inward/record.url?scp=85054055989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054055989&partnerID=8YFLogxK

U2 - 10.1002/cpcb.64

DO - 10.1002/cpcb.64

M3 - Article

C2 - 30265439

AN - SCOPUS:85054055989

JO - Current Protocols in Cell Biology

JF - Current Protocols in Cell Biology

SN - 1934-2500

M1 - e64

ER -