Identification of a histamine release inhibitory factor produced by human mononuclear cells in vitro

R. Alam, J. A. Grant, M. A. Lett-Brown

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Human mononuclear cells (MNC) secrete histamine-releasing factor(s) (HRF) when cultured in vitro. HRF induces the release of histamine and other mediators from basophils and mast cells. We have shown that MNC also produce a histamine release inhibitory factor (HRIF), and that the synthesis is augmented by culture with physiologic concentrations of histamine (10-10 to 10-6 M) and by the mitogen concanavalin A (Con A). HRIF does not affect release initiated by other secretagogues such as allergen, anti-IgE, C5a, Con A, and phorbol myristate acetate. HRIF requires a preincubation with the cells for 5-10 min for maximal inhibition, and this effect is not abolished by washing the cells after the preincubation. The biological activity of HRIF is protease-sensitive, neuraminidase-resistant, and relatively heat-stable. HRIF can be distinguished from HRF by a lower apparent molecular mass (8,000-10,000 D).

Original languageEnglish (US)
Pages (from-to)2056-2062
Number of pages7
JournalJournal of Clinical Investigation
Issue number6
StatePublished - Jan 1 1988


ASJC Scopus subject areas

  • Medicine(all)

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