Identification of a new hemolysin from diarrheal isolate SSU of Aeromonas hydrophila

Tatiana E. Erova, Jian Sha, Amy J. Horneman, Mark A. Borchardt, Bijay K. Khajanchi, Amin A. Fadl, Ashok Chopra

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A clinical strain SSU of Aeromonas hydrophila produces a potent cytotoxic enterotoxin (Act) with cytotoxic, enterotoxic, and hemolytic activities. A new gene, which encoded a hemolysin of 439-amino acid residues with a molecular mass of 49 kDa, was identified. This hemolysin (HlyA) was detected based on the observation that the act gene minus mutant of A. hydrophila SSU still had residual hemolytic activity. The new hemolysin gene (hlyA) was cloned, sequenced, and overexpressed in Escherichia coli. The hlyA gene exhibited 96% identity with its homolog found in a recently annotated genome sequence of an environmental isolate, namely the type strain ATCC 7966 of A. hydrophila subspecies hydrophila. The hlyA gene did not exhibit any homology with other known hemolysins and aerolysin genes detected in Aeromonas isolates. However, this hemolysin exhibited significant homology with hemolysin of Vibrio vulnificus as well as with the cystathionine β synthase domain protein of Shewanella oneidensis. The HlyA protein was activated only after treatment with trypsin and the resulting hemolytic activity was not neutralizable with antibodies to Act. The presence of the hlyA gene in clinical and water Aeromonas isolates was investigated and DNA fingerprint analysis was performed to demonstrate its possible role in Aeromonas virulence.

Original languageEnglish (US)
Pages (from-to)301-311
Number of pages11
JournalFEMS Microbiology Letters
Volume275
Issue number2
DOIs
StatePublished - Oct 2007

Fingerprint

Aeromonas hydrophila
Hemolysin Proteins
Aeromonas
Genes
Cystathionine
Shewanella
Vibrio vulnificus
DNA Fingerprinting
Enterotoxins
Trypsin
Virulence
Genome
Escherichia coli
Amino Acids
Water
Antibodies

Keywords

  • Biochemical characterization
  • DNA fingerprinting
  • Gene distribution
  • Hemolytic activity
  • Sequencing and expression of the hlyA gene

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Identification of a new hemolysin from diarrheal isolate SSU of Aeromonas hydrophila. / Erova, Tatiana E.; Sha, Jian; Horneman, Amy J.; Borchardt, Mark A.; Khajanchi, Bijay K.; Fadl, Amin A.; Chopra, Ashok.

In: FEMS Microbiology Letters, Vol. 275, No. 2, 10.2007, p. 301-311.

Research output: Contribution to journalArticle

Erova, Tatiana E. ; Sha, Jian ; Horneman, Amy J. ; Borchardt, Mark A. ; Khajanchi, Bijay K. ; Fadl, Amin A. ; Chopra, Ashok. / Identification of a new hemolysin from diarrheal isolate SSU of Aeromonas hydrophila. In: FEMS Microbiology Letters. 2007 ; Vol. 275, No. 2. pp. 301-311.
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