Identification of a new north American hantavirus that causes acute pulmonary insufficiency

Thomas Ksiazek, C. J. Peters, P. E. Rollin, S. Zaki, S. Nichol, C. Spiropoulou, S. Morzunov, H. Feldmann, A. Sanchez, A. S. Khan, B. W J Mahy, K. Wachsmuth, J. C. Butler

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

In May 1993, a pulmonary disease syndrome with novel clinical and epidemiologic features was identified in the southwestern United States. Healthy young adults developed a febrile prodrome followed by the rapid onset of often lethal acute respiratory distress. Although an infectious disease was suspected, intensive investigations initially failed to identify the causative agent. Multiple specialized microbiology laboratories at the National Center for Infectious Diseases (Centers for Disease Control and Prevention) applied classic serologic and culture methods as well as recently developed molecular biological techniques to samples collected from field investigations of the patients. Serologic tests detected the presence of an active immune response to a hantavirus. Reverse transcription and polymerase chain reaction amplification of RNA extracted from human tissues used primers designed from sequences of known hantaviruses to demonstrate genomic sequences of a novel hantavirus. Immunohistochemistry showed the presence of hantavirus antigens in the endothelium of lung tissues from patients and provided the final pathogenetic link to this group of viruses. These methods were concordantly positive in virtually all samples available from 18 patients with compatible clinical histories identified between January and July 1993. Test results of control subjects and searches for other agents in identified cases were negative. This newly recognized hantavirus causes a novel syndrome of acute pulmonary edema and shock; the pathogenesis is related to the presence of virus antigens in the pulmonary capillaries. The virus may be an important cause of severe and fatal disease presenting as adult respiratory distress syndrome in otherwise healthy persons.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume52
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Hantavirus
Lung
Viruses
Communicable Diseases
Active Immunity
Southwestern United States
Antigens
Adult Respiratory Distress Syndrome
Serologic Tests
Pulmonary Edema
Centers for Disease Control and Prevention (U.S.)
Microbiology
Lung Diseases
Reverse Transcription
Endothelium
Young Adult
Shock
Fever
Immunohistochemistry
RNA

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Ksiazek, T., Peters, C. J., Rollin, P. E., Zaki, S., Nichol, S., Spiropoulou, C., ... Butler, J. C. (1995). Identification of a new north American hantavirus that causes acute pulmonary insufficiency. American Journal of Tropical Medicine and Hygiene, 52(2), 117-123.

Identification of a new north American hantavirus that causes acute pulmonary insufficiency. / Ksiazek, Thomas; Peters, C. J.; Rollin, P. E.; Zaki, S.; Nichol, S.; Spiropoulou, C.; Morzunov, S.; Feldmann, H.; Sanchez, A.; Khan, A. S.; Mahy, B. W J; Wachsmuth, K.; Butler, J. C.

In: American Journal of Tropical Medicine and Hygiene, Vol. 52, No. 2, 1995, p. 117-123.

Research output: Contribution to journalArticle

Ksiazek, T, Peters, CJ, Rollin, PE, Zaki, S, Nichol, S, Spiropoulou, C, Morzunov, S, Feldmann, H, Sanchez, A, Khan, AS, Mahy, BWJ, Wachsmuth, K & Butler, JC 1995, 'Identification of a new north American hantavirus that causes acute pulmonary insufficiency', American Journal of Tropical Medicine and Hygiene, vol. 52, no. 2, pp. 117-123.
Ksiazek, Thomas ; Peters, C. J. ; Rollin, P. E. ; Zaki, S. ; Nichol, S. ; Spiropoulou, C. ; Morzunov, S. ; Feldmann, H. ; Sanchez, A. ; Khan, A. S. ; Mahy, B. W J ; Wachsmuth, K. ; Butler, J. C. / Identification of a new north American hantavirus that causes acute pulmonary insufficiency. In: American Journal of Tropical Medicine and Hygiene. 1995 ; Vol. 52, No. 2. pp. 117-123.
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