Identification of a new Venezuelan equine encephalitis virus from Brazil

C. H. Calisher, R. M. Kinney, O. de Souza Lopes, D. W. Trent, T. P. Monath, D. B. Francy

Research output: Contribution to journalArticle

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Abstract

Two strains of a recently isolated Venezuelan equine encephalitis (VEE) complex virus from southern Brazil, avirulent for 6- to 8-week-old mice and short-haired guinea pigs, were characterized by biologic, serologic, and biochemical means. They were shown serologically to represent a single, newly recognized variant of subtype I. Two-dimensional polyacrylamide gel electrophoresis (PAGE) of ribonuclease T 1 digests of viral ribonucleic acid showed considerable homology between the genomes of the new variant prototype and variant IA. Three structural proteins were visualized by discontinuous sodium dodecyl sulfate-PAGE (SDS-PAGE). Although the smallest protein of both recent isolates migrates with the capsid proteins of other subtype I viruses, the larger structural proteins of the new variants differ in molecular weight from the E 1 and E 2 envelope glycoproteins of the other subtype I variants. The new isolates produced peptide fragment patterns that were identical to each other, but different from the patterns of other subtype I viruses, following SDS-PAGE of dissociated virions digested with Staphylococcus aureus V8 protease. Since these two isolates were from Culex (Melanoconion) species mosquitoes and from a bat (Carollia perspicillata), we postulate that this is an enzootic VEE virus variant for which the classification IF is suggested.

Original languageEnglish (US)
Pages (from-to)1260-1272
Number of pages13
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume31
Issue number6
StatePublished - 1982

Fingerprint

Venezuelan Equine Encephalitis Viruses
Brazil
Polyacrylamide Gel Electrophoresis
Ribonuclease T1
Viral Structural Proteins
Culex
Peptide Fragments
Electrophoresis, Gel, Two-Dimensional
Capsid Proteins
Culicidae
Sodium Dodecyl Sulfate
Virion
Staphylococcus aureus
Glycoproteins
Guinea Pigs
Proteins
Molecular Weight
Genome
RNA
Viruses

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Calisher, C. H., Kinney, R. M., de Souza Lopes, O., Trent, D. W., Monath, T. P., & Francy, D. B. (1982). Identification of a new Venezuelan equine encephalitis virus from Brazil. American Journal of Tropical Medicine and Hygiene, 31(6), 1260-1272.

Identification of a new Venezuelan equine encephalitis virus from Brazil. / Calisher, C. H.; Kinney, R. M.; de Souza Lopes, O.; Trent, D. W.; Monath, T. P.; Francy, D. B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 31, No. 6, 1982, p. 1260-1272.

Research output: Contribution to journalArticle

Calisher, CH, Kinney, RM, de Souza Lopes, O, Trent, DW, Monath, TP & Francy, DB 1982, 'Identification of a new Venezuelan equine encephalitis virus from Brazil', American Journal of Tropical Medicine and Hygiene, vol. 31, no. 6, pp. 1260-1272.
Calisher CH, Kinney RM, de Souza Lopes O, Trent DW, Monath TP, Francy DB. Identification of a new Venezuelan equine encephalitis virus from Brazil. American Journal of Tropical Medicine and Hygiene. 1982;31(6):1260-1272.
Calisher, C. H. ; Kinney, R. M. ; de Souza Lopes, O. ; Trent, D. W. ; Monath, T. P. ; Francy, D. B. / Identification of a new Venezuelan equine encephalitis virus from Brazil. In: American Journal of Tropical Medicine and Hygiene. 1982 ; Vol. 31, No. 6. pp. 1260-1272.
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