Identification of a new Venezuelan equine encephalitis virus from Brazil

C. H. Calisher, R. M. Kinney, O. de Souza Lopes, D. W. Trent, T. P. Monath, D. B. Francy

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Two strains of a recently isolated Venezuelan equine encephalitis (VEE) complex virus from southern Brazil, avirulent for 6- to 8-week-old mice and short-haired guinea pigs, were characterized by biologic, serologic, and biochemical means. They were shown serologically to represent a single, newly recognized variant of subtype I. Two-dimensional polyacrylamide gel electrophoresis (PAGE) of ribonuclease T 1 digests of viral ribonucleic acid showed considerable homology between the genomes of the new variant prototype and variant IA. Three structural proteins were visualized by discontinuous sodium dodecyl sulfate-PAGE (SDS-PAGE). Although the smallest protein of both recent isolates migrates with the capsid proteins of other subtype I viruses, the larger structural proteins of the new variants differ in molecular weight from the E 1 and E 2 envelope glycoproteins of the other subtype I variants. The new isolates produced peptide fragment patterns that were identical to each other, but different from the patterns of other subtype I viruses, following SDS-PAGE of dissociated virions digested with Staphylococcus aureus V8 protease. Since these two isolates were from Culex (Melanoconion) species mosquitoes and from a bat (Carollia perspicillata), we postulate that this is an enzootic VEE virus variant for which the classification IF is suggested.

Original languageEnglish (US)
Pages (from-to)1260-1272
Number of pages13
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number6
StatePublished - 1982


ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Calisher, C. H., Kinney, R. M., de Souza Lopes, O., Trent, D. W., Monath, T. P., & Francy, D. B. (1982). Identification of a new Venezuelan equine encephalitis virus from Brazil. American Journal of Tropical Medicine and Hygiene, 31(6), 1260-1272.