Identification of a regulated alkaline phosphatase, a cell surface-associated lipoprotein, in Mycobacterium smegmatis

Jordan Kriakov, Sunhee Lee, William R. Jacobs

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Although alkaline phosphatases are common in a wide variety of bacteria, there has been no prior evidence for alkaline phosphatases in Mycobacterium smegmatis. Here we report that transposon insertions in the pst operon, encoding homologues of an inorganic phosphate transporter, leads to constitutive expression of a protein with alkaline phosphatase activity. DNA sequence analysis revealed that M. smegmatis does indeed have a phoA gene that shows high homology to other phoA genes. The M. smegmatis phoA gene was shown to be induced by phosphate starvation and thus negatively regulated by the pst operon. Interestingly, the putative M. smegmatis PhoA has a hydrophobic N-terminal domain which resembles a lipoprotein signal sequence. The M. smegmatis PhoA was demonstrated to be an exported protein associated with the cell surface. Furthermore, immunoprecipitation of PhoA from [14C]acetate-labeled M. smegmatis cell lysates demonstrated that this phosphatase is a lipoprotein.

Original languageEnglish (US)
Pages (from-to)4983-4991
Number of pages9
JournalJournal of Bacteriology
Volume185
Issue number16
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Fingerprint

Mycobacterium smegmatis
Lipoproteins
Alkaline Phosphatase
Operon
Phosphate Transport Proteins
Genes
Protein Sorting Signals
Starvation
DNA Sequence Analysis
Phosphoric Monoester Hydrolases
Immunoprecipitation
Proteins
Acetates
Phosphates
Bacteria

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Identification of a regulated alkaline phosphatase, a cell surface-associated lipoprotein, in Mycobacterium smegmatis. / Kriakov, Jordan; Lee, Sunhee; Jacobs, William R.

In: Journal of Bacteriology, Vol. 185, No. 16, 01.08.2003, p. 4983-4991.

Research output: Contribution to journalArticle

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