Identification of a stable nuclear RNA complementary to the 3'-end flanking sequence of the mouse β-major globin gene

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Abstract

A stable nuclear RNA of approximately 1600 nucleotides (nt) isolated from dimethyl sulfoxide-induced Friend erythroleukemia cells has been characterized. This RNA has been shown to be homologous to a region of unique sequences situated 3' to the mouse β-major globin gene between the poly(A) addition site and a BglII site located 1400 nt further downstream. It is transcribed from the same DNA strand as the β-major globin mRNA, and the amount of this RNA present in the cell is directly proportional to the level of β-globin mRNA. Therefore, the 1600-nt RNA appears to be related to the large primary transcript of the β-major globin gene. We feel that this RNA species is an unusually stable intermediate product of the early processing event which cleaves the primary transcript at the poly(A) addition site. The observed stability and discrete length which are contrary to the expected properties of such a processing intermediate may reflect peculiarities of the transformed state of the Friend erythroleukemia cells.

Original languageEnglish (US)
Pages (from-to)2304-2308
Number of pages5
JournalJournal of Biological Chemistry
Volume261
Issue number5
StatePublished - 1986

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3' Flanking Region
Nuclear RNA
Globins
Genes
RNA
Leukemia, Erythroblastic, Acute
Poly A
Nucleotides
Messenger RNA
Processing
Dimethyl Sulfoxide
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{09c62c7ce5a943489afb0d5cc9508f40,
title = "Identification of a stable nuclear RNA complementary to the 3'-end flanking sequence of the mouse β-major globin gene",
abstract = "A stable nuclear RNA of approximately 1600 nucleotides (nt) isolated from dimethyl sulfoxide-induced Friend erythroleukemia cells has been characterized. This RNA has been shown to be homologous to a region of unique sequences situated 3' to the mouse β-major globin gene between the poly(A) addition site and a BglII site located 1400 nt further downstream. It is transcribed from the same DNA strand as the β-major globin mRNA, and the amount of this RNA present in the cell is directly proportional to the level of β-globin mRNA. Therefore, the 1600-nt RNA appears to be related to the large primary transcript of the β-major globin gene. We feel that this RNA species is an unusually stable intermediate product of the early processing event which cleaves the primary transcript at the poly(A) addition site. The observed stability and discrete length which are contrary to the expected properties of such a processing intermediate may reflect peculiarities of the transformed state of the Friend erythroleukemia cells.",
author = "Rabek, {J. P.} and John Papaconstantinou",
year = "1986",
language = "English (US)",
volume = "261",
pages = "2304--2308",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

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TY - JOUR

T1 - Identification of a stable nuclear RNA complementary to the 3'-end flanking sequence of the mouse β-major globin gene

AU - Rabek, J. P.

AU - Papaconstantinou, John

PY - 1986

Y1 - 1986

N2 - A stable nuclear RNA of approximately 1600 nucleotides (nt) isolated from dimethyl sulfoxide-induced Friend erythroleukemia cells has been characterized. This RNA has been shown to be homologous to a region of unique sequences situated 3' to the mouse β-major globin gene between the poly(A) addition site and a BglII site located 1400 nt further downstream. It is transcribed from the same DNA strand as the β-major globin mRNA, and the amount of this RNA present in the cell is directly proportional to the level of β-globin mRNA. Therefore, the 1600-nt RNA appears to be related to the large primary transcript of the β-major globin gene. We feel that this RNA species is an unusually stable intermediate product of the early processing event which cleaves the primary transcript at the poly(A) addition site. The observed stability and discrete length which are contrary to the expected properties of such a processing intermediate may reflect peculiarities of the transformed state of the Friend erythroleukemia cells.

AB - A stable nuclear RNA of approximately 1600 nucleotides (nt) isolated from dimethyl sulfoxide-induced Friend erythroleukemia cells has been characterized. This RNA has been shown to be homologous to a region of unique sequences situated 3' to the mouse β-major globin gene between the poly(A) addition site and a BglII site located 1400 nt further downstream. It is transcribed from the same DNA strand as the β-major globin mRNA, and the amount of this RNA present in the cell is directly proportional to the level of β-globin mRNA. Therefore, the 1600-nt RNA appears to be related to the large primary transcript of the β-major globin gene. We feel that this RNA species is an unusually stable intermediate product of the early processing event which cleaves the primary transcript at the poly(A) addition site. The observed stability and discrete length which are contrary to the expected properties of such a processing intermediate may reflect peculiarities of the transformed state of the Friend erythroleukemia cells.

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