Identification of a T-cell epitope that is globally conserved among outer membrane proteins (OMPs) OMP7, OMP8, and OMP9 of anaplasma marginale strains and with OMP7 from the A. marginale subsp. centrale vaccine strain

James R. Deringer, Elkin Gustavo Forero Becerra, Massaro W. Ueti, Joshua E. Turse, James E. Futse, Susan M. Noh, Guy H. Palmer, Wendy C. Brown

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Within the protective outer membrane (OM) fraction of Anaplasma marginale, several vaccine candidates have emerged, including a family of OM proteins (OMPs) 7 to 9, which share sequence identity with each other and with the single protein OMP7 in the vaccine strain A. marginale subsp. centrale. A. marginale OMPs 7 to 9 are logical vaccine candidates because they are surface exposed, present in the OM immunogen and protective cross-linked OM proteins, recognized by immune serum IgG2 and T cells in cattle immunized with OM, and recognized by immune serum IgG2 from cattle immunized with the A. centrale vaccine strain. We report the identification of a globally conserved 9-amino-acid T-cell epitope FLLVDDAI/VV shared between A. centrale vaccine strain OMP7 and the related A. marginale OMPs 7 to 9, where position 8 of the peptide can be isoleucine or valine. The epitope is conserved in American A. marginale strains, in the Australia Gypsy Plains strain, and in multiple field isolates from Ghana. This epitope, together with additional T-cell epitopes that are present within these proteins, should be considered for inclusion in a multivalent vaccine for A. marginale that can provide protection against disease caused by globally distributed bacterial strains.

Original languageEnglish (US)
Article numbere00406
JournalClinical and Vaccine Immunology
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

Anaplasma marginale
T-Lymphocyte Epitopes
Membrane Proteins
Vaccines
Membranes
Immune Sera
Epitopes
Immunoglobulin G
Ghana
T-cells
Isoleucine
Valine
Proteins
T-Lymphocytes
Amino Acids
Peptides

Keywords

  • Anaplasma centrale
  • Anaplasma marginale
  • Outer membrane protein 7
  • Outer membrane protein 8
  • Outer membrane protein 9
  • T-cell epitopes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Identification of a T-cell epitope that is globally conserved among outer membrane proteins (OMPs) OMP7, OMP8, and OMP9 of anaplasma marginale strains and with OMP7 from the A. marginale subsp. centrale vaccine strain. / Deringer, James R.; Forero Becerra, Elkin Gustavo; Ueti, Massaro W.; Turse, Joshua E.; Futse, James E.; Noh, Susan M.; Palmer, Guy H.; Brown, Wendy C.

In: Clinical and Vaccine Immunology, Vol. 24, No. 1, e00406, 01.01.2017.

Research output: Contribution to journalArticle

Deringer, James R. ; Forero Becerra, Elkin Gustavo ; Ueti, Massaro W. ; Turse, Joshua E. ; Futse, James E. ; Noh, Susan M. ; Palmer, Guy H. ; Brown, Wendy C. / Identification of a T-cell epitope that is globally conserved among outer membrane proteins (OMPs) OMP7, OMP8, and OMP9 of anaplasma marginale strains and with OMP7 from the A. marginale subsp. centrale vaccine strain. In: Clinical and Vaccine Immunology. 2017 ; Vol. 24, No. 1.
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