Identification of an immunodominant T cell epitope on cholera toxin

Yingzi Cong, Hazel R. Bowdon, Charles O. Elson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cholera toxin (CT), the enterotoxin of Vibrio cholerae, is a potent mucosal immunogen as well as a strong mucosal adjuvant to related and unrelated antigens. The mucosal immune response to CT is T cell dependent and MHC class II restricted. The epitopes on CT recognized by T cells have not been identified. The purpose of this study was to determine the fine specificity of T cell recognition of both the CT A subunit (CT-A) and the CT B subunit (CT-B) by using a range of synthetic peptides. After immunization with CT-B or CT-A in CFA subcutaneously, the peripheral lymph node T cells were stimulated with different synthetic peptides in vitro. The peptide specificity of T cell recognition was identified by assaying T cell proliferation and interleukin-3 production. T cells from C57BL/6 (H-2b) high responder mice recognized one immunodominant epitope (peptide 89-100) and one weak epitope (peptide 31-50) on CT-B and two epitopes (peptide 21-39 and 180-194) on CT-A. The immunization of C57BL/6 mice with synthetic immunodominant CT-B peptide 89-100 induced T cell immunity to the pentameric CT-B. Induction of tolerance to CTB peptide 89-100 by i.v. injection in high responder C57BL/6 mice induced unresponsiveness to mucosal immunization with CT, compatible with an immunodominant role for this T cell epitope.

Original languageEnglish (US)
Pages (from-to)2587-2594
Number of pages8
JournalEuropean Journal of Immunology
Volume26
Issue number11
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Immunodominant Epitopes
T-Lymphocyte Epitopes
Cholera Toxin
T-Lymphocytes
Peptides
Epitopes
Immunization
Inbred C57BL Mouse
T-Cell Antigen Receptor Specificity
Peptide T
Mucosal Immunity
Vibrio cholerae
Interleukin-3
Immunity

Keywords

  • Cholera toxin
  • Epitope
  • T cell
  • Tolerance

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of an immunodominant T cell epitope on cholera toxin. / Cong, Yingzi; Bowdon, Hazel R.; Elson, Charles O.

In: European Journal of Immunology, Vol. 26, No. 11, 1996, p. 2587-2594.

Research output: Contribution to journalArticle

Cong, Yingzi ; Bowdon, Hazel R. ; Elson, Charles O. / Identification of an immunodominant T cell epitope on cholera toxin. In: European Journal of Immunology. 1996 ; Vol. 26, No. 11. pp. 2587-2594.
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