Identification of antigens recognized by T cells in human leishmaniasis: Analysis of T-cell clones by immunoblotting

P. C. Melby, D. L. Sacks

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Immunity in human leishmaniasis is mediated by sensitized T lymphocytes; however, the antigens involved in eliciting this immunity have not been defined. We describe the generation of human T-lymphocyte clones derived from two patients with healed leishmaniasis. By use of one- and two-dimensional cellular immunoblotting techniques, we directly identified the parasite antigens recognized by these clones. To our knowledge, these are the first leishmanial antigens identified to which CD4+, gamma interferon-producing T cells from immune individuals have been shown to respond, and the strategy may be of general use for the identification of antigens involved in immunity in this disease.

Original languageEnglish (US)
Pages (from-to)2971-2976
Number of pages6
JournalInfection and immunity
Volume57
Issue number10
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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